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F1757

Sigma-Aldrich

Fumarase from porcine heart

ammonium sulfate suspension, ≥300 units/mg protein (biuret)

Sinónimos:

L-Malate hydrolyase, Fumarate hydratase

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About This Item

Número de CAS:
9032-88-6
Comisión internacional de enzimas:
4.2.1.2 (BRENDA, IUBMB)
EC Number:
232-887-7
MDL number:
MFCD00131111
UNSPSC Code:
12352204
NACRES:
NA.54

form

ammonium sulfate suspension

specific activity

≥300 units/mg protein (biuret)

foreign activity

malic dehydrogenase ≤0.1%

storage temp.

2-8°C

General description

Fumarase (FH in human) is a well-known tricarboxylic-acid-cycle enzyme found in both the cytoplasm and mitochondria of all eukaryotes.
Fumarase is mapped to human chromosome 1q43. Fumarase comprises of three domains D1, D2 and D3 and belongs to class II fumarase family.

Application

Fumarase from porcine heart has been used:
  • as a positive control in isocitrate dehydrogenase and α-ketoglutarate dehydrogenase assay
  • for immobilized enzyme based stability assay
  • in fumarate hydratase assays in human lung carcinoma cells (A549)

Fumarase is used as a protein calibration standard in the purification of intact DNA polymerase a/primase from mouse cells.

Biochem/physiol Actions

Fumarase catalyzes the reversible hydration of fumarate to malate. In its mitochondrial form, fumarate is involved in the Krebs Cycle, while the cytosolic form is involved in amino acid metabolism.
Fumarase is essential for regulation of cellular metabolism. Fumarase elicits protective function from double-strand breaks (DSBs) induced by infrared radiation. Loss of function mutations in the fumarase gene is implicated in hereditary leiomyomatosis and renal cell cancer (HLRCC)-syndrome.

Unit Definition

One unit will convert 1.0 μmole of L-malate to fumarate per min at pH 7.6 at 25 °C.

Physical form

Suspension in 3.2 M (NH4)2SO4, 0.05 M KH2PO4, pH 7.5, 0.014 M 2-mercaptoethanol

Preparation Note

Isolated by the method of Beeckmans, S., and Kanarek, L., Eur. J. Biochem., 78, 437 (1977). Affinity purified.

pictograms

Exclamation mark
GHS07

signalword

Warning

hcodes

H317

Hazard Classifications

Skin Sens. 1

Storage Class

10 - Combustible liquids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

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Fumarase: a paradigm of dual targeting and dual localized functions
Yogev O, et al.
FEBS Journal, 278(22), 4230-4242 (2011)
Fumarase Deficiency: A Safe and Potentially Disease Modifying Effect of High Fat/Low Carbohydrate Diet
Ryder B, et al.
JIMD Reports, 40(3), 77?83-77?83 (2018)
Study of an enzyme membrane reactor with immobilized fumarase for production of L-malic acid
Giorno L, et al.
Biotechnology and Bioengineering, 72(1), 77-84 (2001)
Identification of the catalytic mechanism and estimation of kinetic parameters for fumarase
Mescam M, et al.
The Journal of Biological Chemistry, jbc-M110 (2011)
Intact DNA Polymerase a/Primase from Mouse Cells
Journal of Biochemistry, 264, 19407-19415 (1989)
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Artículos

How to Work with Enzymes Supplied as Ammonium Sulfate Suspensions

Instructions for working with enzymes supplied as ammonium sulfate suspensions

Protocolos

Enzymatic Assay of Fumarase

Enzymatic Assay of Fumarase

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