GenElute™-E does not introduce biases that some “bind-wash-elute” technologies can add because the technology separates by size, rather than by what binds and what is released.
EC400
GenElute™-E Single Spin Cell Culture DNA Kit
Reagents and consumables for various cell culture sample types. 10, 50 or 250 purifications.
Sinónimos:
GenElute™-E Cell Culture DNA Kit
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| A ustedes/SKU | Disponibilidad | Precio |
|---|---|---|
10 reactions | Comprobar disponibilidad del carrito | $56.70 |
50 reactions | Comprobar disponibilidad del carrito | $245.00 |
250 reactions | Comprobar disponibilidad del carrito | $1,080.00 |
Acerca de este artículo
NACRES:
NA.52
UNSPSC Code:
41105600
Servicio técnico
¿Necesita ayuda? Nuestro equipo de científicos experimentados está aquí para ayudarle.
Permítanos ayudarlepurified by
(Single-spin negative chromotography), (Time: 30 minutes or less)
feature
Compatible Application (Suitable for most common downstream applications, including genotyping, PCR, and NGS), Intended use (For purification of genomic DNA from cell lines), Typical/expected yield (Varies by sample. Please reference user guide for more information.)
greener alternative product characteristics
Waste Prevention
Designing Safer Chemicals
Learn more about the Principles of Green Chemistry.
sustainability
Greener Alternative Product
technique(s)
DNA purification: suitable
input
cells
test parameters
: 3 min hands on time
greener alternative category
storage temp.
2-8°C
General description
GenElute™-E purification kits provide a simple alternative for DNA purification. Compared with traditional methods, GenElute™-E offers:
Traditional silica-based, bind-wash-elute purification kits require multiple wash steps to remove impurities from the spin columns. These steps increase the risk of cross contamination, subject the DNA to centrifugation sheering forces, and introduce chaotropic salts that can carry over into the final sample, inhibiting downstream applications.
GenElute™-E kits employ size exclusion negative chromatography to separate large nucleic acid molecules from smaller protein, lipid, and ionic components in cell, tissue, blood, and other samples. Single-spin columns efficiently absorb and retain cellular debris and sample contaminants while allowing nucleic acids to pass through, reducing the number of steps and plastic materials required for purification. This novel method for high-quality purification is made possible by our innovative SmartLyse® Protease, which enables fast and efficient lysis of a wide range of sample types.
Isolate nucleic acid in a fraction of time compared to traditional silica bind-wash-elute procedures. Simply mix sample with lysis components and incubate according to kit protocol, pipette sample onto the spin column, and centrifuge directly into a collection tube. Cellular debris and contaminants remain bound in the column to be discarded, while purified DNA is ready to be used in downstream applications or stored.
- Isolation of DNA in under an hour with minimal sample handling
- Better purity, leading to improved performance in PCR and other applications
- Significant reduction in plastic and hazardous chemical waste
Traditional silica-based, bind-wash-elute purification kits require multiple wash steps to remove impurities from the spin columns. These steps increase the risk of cross contamination, subject the DNA to centrifugation sheering forces, and introduce chaotropic salts that can carry over into the final sample, inhibiting downstream applications.
GenElute™-E kits employ size exclusion negative chromatography to separate large nucleic acid molecules from smaller protein, lipid, and ionic components in cell, tissue, blood, and other samples. Single-spin columns efficiently absorb and retain cellular debris and sample contaminants while allowing nucleic acids to pass through, reducing the number of steps and plastic materials required for purification. This novel method for high-quality purification is made possible by our innovative SmartLyse® Protease, which enables fast and efficient lysis of a wide range of sample types.
Isolate nucleic acid in a fraction of time compared to traditional silica bind-wash-elute procedures. Simply mix sample with lysis components and incubate according to kit protocol, pipette sample onto the spin column, and centrifuge directly into a collection tube. Cellular debris and contaminants remain bound in the column to be discarded, while purified DNA is ready to be used in downstream applications or stored.
We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product is aligned with "Waste prevention" and "Designing safer chemicals". Click here for more information.
Application
The GenElute™-E Single Spin Cell Culture DNA Kit purifies genomic DNA from common cultured cell lines. DNA from up to 2 x 10^6 fresh or frozen cells can purified using a standard protocol, without the need for additional optimizations.
The initial lysis step takes place under physiological conditions where the enzyme activity of proteases is generally increased. SmartLyse® Protease contains a unique combination of several active enzymes with an expanded substrate specificity. This eliminates the requirement for extended, overnight incubations, which are required in many conventional protocols.
Purified genomic DNA is eluted in Tris buffer, pH 7.8 and can immediately be used for most common downstream applications, including PCR, genotyping, NGS, and others. Final yield is equal or better than silica-based methods depending on sample type. GenElute™-E purified genomic DNA preparations commonly show an A260/280 ratio of around 1.8.
The initial lysis step takes place under physiological conditions where the enzyme activity of proteases is generally increased. SmartLyse® Protease contains a unique combination of several active enzymes with an expanded substrate specificity. This eliminates the requirement for extended, overnight incubations, which are required in many conventional protocols.
Purified genomic DNA is eluted in Tris buffer, pH 7.8 and can immediately be used for most common downstream applications, including PCR, genotyping, NGS, and others. Final yield is equal or better than silica-based methods depending on sample type. GenElute™-E purified genomic DNA preparations commonly show an A260/280 ratio of around 1.8.
Features and Benefits
Fast and efficient purification: 10-minute lysis and minimal hands-on, preparation stepsHigher quality DNA: negative chromotography-based purification reduces carryover of PCR inhibitors and other contaminents Reduced environmental impact: 55% less plastic waste, as well as a significant reduction in hazardous chemicals
Preparation Note
Store enzymes at 2-8°C for up to 12 months. All other components should be stored at room temperature.
Other Notes
We believe that green chemistry will contribute to a better tomorrow. With a growing portfolio of greener alternatives, there are now more choices to reduce the ecological impact of your research while still delivering quality and efficacy so your results are not compromised. GenElute™-E purification kits avoid the use of hazardous wash buffers typically employed in silica-based purification kits, significantly reducing chemical waste. The single-spin protocol also limits the number of collection steps, reducing plastic waste by 55% compared to equivalent bind-wash-elute methods. GenElute™-E is designated a Greener Alternative Product based on the "Prevention" and "Designing Safer Chemicals" qualifications of The 12 Principles of Green Chemistry.GenElute™-E kits also adhere to the principles of SMASH Packaging, our global initiative to improve the sustainability through the reduction of packaging materials, the increased use of sustainable alternatives, and improved recyclability. Packaging is FCS-marked for sustainable forestry practices and produced from greater than 70% recycled content.
Legal Information
GenElute is a trademark of Sigma-Aldrich Co. LLC
SmartLyse is a registered trademark of Merck KGaA, Darmstadt, Germany
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Este artículo | |||
|---|---|---|---|
| technique(s) DNA purification: suitable | technique(s) DNA purification: suitable | technique(s) DNA purification: suitable | technique(s) DNA purification: suitable |
| test parameters : 3 min hands on time | test parameters : 3 min hands on time, sample volume: 20 mg | test parameters : 3 min hands on time, sample volume: 60 μL blood or 2-5 dried blood spots | test parameters : 3 min hands on time, sample volume: 10-50 mg |
| greener alternative category | greener alternative category | greener alternative category , Aligned | greener alternative category , Aligned |
| storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-8°C |
| greener alternative product characteristics Waste Prevention | greener alternative product characteristics Waste Prevention | greener alternative product characteristics Waste Prevention | greener alternative product characteristics Waste Prevention |
| input cells | input tissue (fresh or frozen) | input blood (liquid or dried) | input plant tissue (leaves, blossoms, fruits, roots, flour and seeds) |
Solo componentes del kit
Referencia del producto
Descripción
- Cell Lysis Buffer
- SmartLyse® C Protease
- Clearing Solution C
- RNase A Cell
- 1x Tris Buffer
- Spin Columns
signalword
Danger
Hazard Classifications
Aquatic Chronic 3 - Eye Dam. 1 - Flam. Liq. 2 - Met. Corr. 1 - Resp. Sens. 1
Clase de almacenamiento
3 - Flammable liquids
wgk
WGK 3
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Does the technology introduce any bias into the sample?
1 answer-
Helpful?
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Genelute-e single spin DNA and RNA purification kits use negative chromatographty to isolate nucleic acids. Can you explain how this approach simplifies workflows?
1 answer-
Instead of optimizing the bind, wash, and release steps in conventional silica-based spin purification preps, the technology focuses on a separation by performing a single step fractionation based on the size of the biomolecules, which results in depleted impurities.
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What is the composition of the lysis buffer and clearing buffer after flowing through the resin?
1 answer-
The presence of EDTA, SDS, or excess salt can affect my PCR/ sequencing reaction. The lysis buffer information is proprietary, but we can say it is free of chaotropic salts. The resins are desalting resins so EDTA, SDS, and salts are depleted.
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Do we know how stable the purified DNA is through several freeze-thaw cycles?
1 answer-
This will fluctuate due to sample variability (sample collection, concentration, fragment length, sequence [GC content], storage before isolation, etc.). However, the sample is buffer exchanged into a standard storage buffer that is included in the kit (1X TE).
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Has testing been conducted on the GenElute™-E kits to determine if they are capable of retrieving mitochondrial DNA?
1 answer-
The GenElute™-E kits have not been tested for mtDNA. It's important to note that GenElute-E is not recommended for nucleotide isolation smaller than 50bp in size.
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