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E6779

Millipore

EZview™ Red Anti-HA Affinity Gel

Sinónimos:

Anti-HA

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1 ML
$726.00
5 X 1 ML
$2,910.00

$726.00

Fecha estimada de envío30 de mayo de 2025

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1 ML
$726.00
5 X 1 ML
$2,910.00

About This Item

Código UNSPSC:
12352203
NACRES:
NA.56

$726.00

Fecha estimada de envío30 de mayo de 2025

caducidad

1 yr at 2‑8 °C

clases químicas de analitos

proteins (hemagglutinin)

técnicas

affinity chromatography: suitable
immunoprecipitation (IP): suitable

Matriz

crosslinked agarose

capacidad

≥0.4 mg/mL, gel binding capacity (HA-tagged fusion protein)

temp. de almacenamiento

2-8°C

Categorías relacionadas

Descripción general

EZview Red Anti-HA Affinity Gel is a red colored Anti-HA agarose affinity gel that contains anti-HA monoclonal antibody which is covalently attached to crosslinked agarose beads. The HA tag has the sequence YPYDVPDYA and derives from the hemagglutinin (HA) protein. This sequence occurs at amino acid residues 98-106 of the full-length HA protein. The HA tag has a high presence of charged amino acids, which enhances its likelihood of strong antibody recognition.

Especificidad

Binding specificity: HA-tag peptide (N-Tyr-Pro-Tyr-Asp-Val-Pro-Asp-Tyr-Ala-C). It recognizes reduced forms of HA-tagged proteins and is reactive with N or C-terminal HA-tagged fusion proteins.

Aplicación

EZview™ Red Anti-HA Affinity Gel has been used for immunoblot analysis[1] and immunoprecipitation assay.[2]
Suitable for Affinity capture (immunoprecipitation) of N- or C- terminal HA-tagged fusion proteins from E. coli or mammalian cell.
When performing small-scale affinity capture, such as immunoprecipitation, the affinity matrix is difficult to see in the microcentrifuge tubes. Accidental aspiration of the resin leads to quantitative variability in results. The EZview™ Red Affinity Gels greatly reduces the risk of pellet loss. EZview resins perform as well as conventional non-colored affinity gels, but allow the user to easily differentiate pellet from supernatant. This correlates to more accurate data because less protein is lost.

Características y beneficios

  • Increased visibility - Red color reduces risk of incidental aspiration
  • Improved recovery of target protein by reduced accidental loss
  • Higher reproducibility - More consistent yields

Otras notas

The affinity gel contains affinity purified anti-HA mouse monoclonal antibody (Clone HA-7) covalently attached to beaded agarose. The HA tag corresponds to amino acid residues 98-106 (YPYDVPDYA) of human influenza virus hemagglutinin (HA).

Forma física

1:1 (v/v) suspension in PBS containing 50% glycerol and 15 ppm Kathon

Información legal

EZview is a trademark of Sigma-Aldrich Co. LLC

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Referencia del producto
Descripción
Precios

08168

Timestrip Plus™ 8 °C

Código de clase de almacenamiento

10 - Combustible liquids

Clase de riesgo para el agua (WGK)

WGK 3

Punto de inflamabilidad (°F)

Not applicable

Punto de inflamabilidad (°C)

Not applicable

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Certificados de análisis (COA)

Lot/Batch Number
0000373135
0000360953
0000353120
0000343116
0000307406

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