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MilliporeSigma

E1645

Sigma-Aldrich

Anti-Endothelin antibody produced in rabbit

whole antiserum

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About This Item

MDL number:
UNSPSC Code:
12352203
NACRES:
NA.41

biological source

rabbit

conjugate

unconjugated

antibody form

whole antiserum

antibody product type

primary antibodies

clone

polyclonal

contains

15 mM sodium azide

species reactivity

human, rat, pig

technique(s)

indirect ELISA: 1:30,000
radioimmunoassay: 1:10,000 using 125I-endothelin-1, human

UniProt accession no.

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... EDN1(1906)
rat ... Edn1(24323)

General description

Endothelin consists of a family of potent vasoconstrictor peptides, which include four structurally related isoforms, ET-1, ET-2, ET-3 and vasointestinal contractor (VIC, β-ET).

Specificity

Reacts with endothelin-1 (ET-1), (human, porcine), endothelin-2 (ET-2), (human), and endothelin-3 (ET-3), (human, rat). Cross-reactivity is seen with sarafotoxin S6c, but not with big-endothelin 38 (human), big-endothelin 39 (rat) or atrial natriuretic peptide (human). There is minimal cross-reaction with angiotensin I (human).

Immunogen

synthetic human endothelin-1 conjugated to KLH

Application

Anti-Endothelin antibody produced in rabbit is suitable for immunodetection of endothelins (ET-1, ET-2, and ET-3) in various immunoassays including RIA (titer of 1:30,000) and ELISA (titer of 1:10,000). Antibodies that react specifically with endothelins (ET-1, ET-2, and ET-3) may be used to detect endothelins in tissue extracts and biological fluids as well as study differential tissue expression, and intracellular localization of the endothelin isoforms in the periphery and central nervous system.

Biochem/physiol Actions

Endothelins (ET) show potent constrictor activity in vascular and non-vascular smooth muscle. ETs can mediate biological effects in cells and tissues, and have been shown to bind to an ET receptor in the lung, kidney, heart, and liver. The biological actions of ETs are mediated by activation of phospholipase C through specific G protein-coupled receptors.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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I Saenz de Tejada et al.
The American journal of physiology, 261(4 Pt 2), H1078-H1085 (1991-10-01)
The localization, synthesis, and activity of endothelin and the receptor types mediating its effects in penile corpus cavernosum were investigated in whole tissue and in cultured cells derived from this tissue. With immunocytochemistry, utilizing an antiendothelin 1 (ET-1) monoclonal antibody
T Sakurai et al.
Trends in pharmacological sciences, 13(3), 103-108 (1992-03-01)
Following the first report on the identification of endothelin (ET), an increasing body of work has accumulated on this endothelium-derived 21-amino acid vasoconstrictor peptide. Subsequently, the existence of three distinct isoforms of ET, designated ET-1, ET-2 and ET-3, was predicted
A Inoue et al.
Proceedings of the National Academy of Sciences of the United States of America, 86(8), 2863-2867 (1989-04-01)
Three distinct human endothelin-related genes were cloned by screening a genomic DNA library under a low hybridization stringency with a synthetic oligonucleotide probe encoding a portion of the endothelin sequence. Genomic Southern blot analysis with the same oligonucleotide probe showed

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