DUO96001
Duolink® flowPLA Multicolor Reagent Pack
Duolink® PLA kit for Flow Cytometry Multiplexing
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About This Item
Productos recomendados
Quality Level
product line
Duolink®
technique(s)
flow cytometry: suitable
immunofluorescence: suitable
multiplexing: suitable
proximity ligation assay: suitable
shipped in
dry ice
storage temp.
−20°C
Categorías relacionadas
Specificity
Duolink® flowPLA Multicolor Reagent Pack contains all the necessary reagents to perform the amplification and detection of bound custom PLA probes generated using Duolink® PLA Multicolor Probemaker Kits. Sufficient components are provided for approximately 40 tests at 100 μL reaction volume.
Application
Duolink® Multicolor flow PLA Reagent Pack Kits used with Duolink® PLA Multicolor Probemaker Kits will enable Multiplexing of up to 4 protein events with sensitive detection of proteins, protein-protein interactions, and protein modifications within cell populations by flow cytometry. To perform a Duolink® flowPLA experiment, you will need fixed and permeabilized suspended cells, and at least two primary antibodies that specifically recognize your proteins of interest. Analysis is carried out using standard flow cytometry assay equipment. User must provide a fixed cell suspension and primary antibodies.
Application Note
Primary antibodies are needed. Test your primary antibodies (IgG-class, mono- or polyclonal) in a standard immunofluorescence (IF), immunohistochemistry (IHC) or immunocytochemistry (ICC) assay to determine the optimal fixation, blocking, and titer conditions. Duolink® in situ reagents are suitable for use on fixed cells, cytospin cells, cells grown on slide, formalin-fixed, paraffin embedded (FFPE), or tissue (fresh or frozen). Flow validated antibodies are recommended. It is also highly recommended that users purchase our Duolink(insert registration symbol) Compensation Beads (make title a hyperlink to beads products) to inform experimental design, instrument settings, and assist with compensation parameters.
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Features and Benefits
- Visualize and detect multiple proteins events in one experiment
- No overexpression or genetic manipulation required
- High specificity (fewer false positives)
- Detection of low abundant proteins due to rolling circle amplification
- Relative quantification possible
- No special equipment needed
- Faster and more simple than FRET
- Publication-ready results
Components
- 5x Multicolor PLA Ligation Buffer (DUO86002)
- 5x Amplification Buffer (DUO82050)
- 5x Multicolor FlowPLA Detection Buffer (DUO86003)
- 1x Ligase (DUO82025)
- 1x Polymerase (DUO82026)
- 1x Blocking Solution (DUO82007)
- Duolink Probemaker PLA Probe Diluent (DUO82036)
See datasheet for more information.
Legal Information
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Warning
hcodes
Hazard Classifications
Aquatic Chronic 2 - Skin Sens. 1
Storage Class
10 - Combustible liquids
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