D9909
Dextran Sucrase from Leuconostoc mesenteroides
lyophilized powder, ≥100 units/mg protein
Iniciar sesiónpara Ver la Fijación de precios por contrato y de la organización
Seleccione un Tamaño
10 UNITS
$405.84
$405.84
Precio de catálogo$456.00Ahorre 11 %Disponible para envío el02 de abril de 2025Detalles
Seleccione un Tamaño
Cambiar Vistas
10 UNITS
$405.84
About This Item
Número de CAS:
Número MDL:
Código UNSPSC:
12352204
NACRES:
NA.54
$405.84
Precio de catálogo$456.00Ahorre 11 %Disponible para envío el02 de abril de 2025Detalles
Productos recomendados
origen biológico
bacterial (Leuconostoc mesenteroides)
Nivel de calidad
Formulario
lyophilized powder
actividad específica
≥100 units/mg protein
composición
Protein, ~15% Lowry
solubilidad
H2O: soluble 0.9-1.1 mg/mL, clear to slightly hazy, colorless to light yellow
temp. de almacenamiento
−20°C
Descripción general
Dextran Sucrase from Leuconostoc mesenteroides belongs to glycoside hydrolase family 70 (GH70). It functions through a retaining mechanism and uses two catalytic acidic residues. Dextran sucrase has a dextran binding site in the C-terminal domain.[1]
Aplicación
Dextran Sucrase from Leuconostoc mesenteroides has been used:
- in immobilization on shirasu porous membrane (SPG) for dextran production[2]
- in the enzymatic synthesis of dextran nanoparticles at various pH range[1]
- in the immobilization with hydroxyapatite for dextran production[3]
Dextran sucrase from Leuconostoc mesenteroides has been used in a study to investigate the functional and structural characterization of α-(1→2) branching sucrase derived from DSR-E glucansucrase. Dextran sucrase from Leuconostoc mesenteroides has also been used in a study to investigate the bioengineering of Leuconostoc mesenteroides glucansucrases.
The enzyme from Sigma has been used to prepare immobilized sphere for the production of dextran from sucrose.[4]
Acciones bioquímicas o fisiológicas
Dextransucrases are glucansucrases that are able to produce dextran, a glucose polymer linked mainly through α1-6 bonds. However, α1-3, α1-6, α1-4 and α1-2 bonds are also found, in both the main chain and the branching linkages.[5] The peptide has approximately 1600 amino acids. The aspartic acid in position 551 is essential for catalytic activity, while glutamic acid 589 and aspartic acid 662 complement the catalytic triad.[6] The activity of dextransucrase is decreased by EDTA, and is restored by the addition of calcium ions. Zinc, cadmium, lead, mercury and copper ions are inhibitory to various degrees.[7]
Calidad
Chromatographically purified
Definición de unidad
One unit will liberate 1.0 μmole of fructose per min at 37 °C, pH 5.4.
Forma física
Lyophilized powder containing dextran, MES buffer salts and CaCl2
Código de clase de almacenamiento
11 - Combustible Solids
Clase de riesgo para el agua (WGK)
WGK 3
Punto de inflamabilidad (°F)
Not applicable
Punto de inflamabilidad (°C)
Not applicable
Equipo de protección personal
Eyeshields, Gloves, type N95 (US)
Elija entre una de las versiones más recientes:
Certificados de análisis (COA)
Lot/Batch Number
¿No ve la versión correcta?
Si necesita una versión concreta, puede buscar un certificado específico por el número de lote.
¿Ya tiene este producto?
Encuentre la documentación para los productos que ha comprado recientemente en la Biblioteca de documentos.
Los clientes también vieron
Hongbin Zhang et al.
Sheng wu gong cheng xue bao = Chinese journal of biotechnology, 25(12), 2022-2028 (2010-04-01)
We optimized the medium for recombinant dextransucrase expression in engineering strain Escherichia coli BL21 (DE3)/pET28-dexYG by an Orthogonal experiment. After the medium had been decided, we studied the effect of temperature, sucrose concentration and pH value on the yield. The
Bioengineering of Leuconostoc mesenteroides glucansucrases that gives selected bond formation for glucan synthesis and/or acceptor-product synt
Kang, H., et al.
RNA, 59, 4148-4155 (2011)
Marie-Sophie Bounaix et al.
FEMS microbiology letters, 311(1), 18-26 (2010-08-21)
The study of exopolysaccharide production by heterofermentative sourdough lactic acid bacteria has shown that Weissella strains isolated from sourdoughs produce linear dextrans containing α-(1→6) glucose residues with few α-(1→3) linkages from sucrose. In this study, several dextran-producing strains, Weissella cibaria
Ah-Rum Yi et al.
Journal of microbiology and biotechnology, 19(8), 829-835 (2009-09-08)
A dextransucrase (LcDS) gene from Leuconostoc citreum HJ-P4 has been amplified and cloned in E. coli. The LcDS gene consists of 4,431 nucleotides encoding 1,477 amino acid residues sharing 63-98% of amino acid sequence identities with other known dextransucrases from
Enzymatically synthesized dextran nanoparticles and their use as carriers for nutraceuticals
Semyonov D, et al.
Food & Function, 5(10), 2463-2474 (2014)
Active Filters
Nuestro equipo de científicos tiene experiencia en todas las áreas de investigación: Ciencias de la vida, Ciencia de los materiales, Síntesis química, Cromatografía, Analítica y muchas otras.
Póngase en contacto con el Servicio técnico
