Lu2

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA.

• Código UNSPSC: 41106514

Propiedades

• origen biológico: Mouse x Mouse Hybridoma
• envase: tube of 5 μg 99043003-DNA-5UG; pkg of vial of cells 99043003-1VL
• ImmunoDon: BALB/c x C57 Bl/6
• Mieloma: P3x63Ag8.653
• Reactividad: Anti-DR11
• técnicas: cell culture | mammalian: suitable
• isotipo: IgM
• Condiciones de envío: dry ice
• temp. de almacenamiento: −196°C

Descripción

Origen línea celular

Mouse x Mouse hybridoma

Descripción línea celular

The cell line Lu2 has been established by fusion of the myeloma P3x63Ag8.653 and spleen cells from BALB/c x C57Bl/6 female mice. Mice received a single i.p. injection with the B cell line LuMar expressing HLA A2.3/B18.5/C5/DR3,11.and B. pertussis three days before fusion. The cell line has been eradicated from mycoplasma using Cibrobay.

Inmunógeno

B cell line LuMar

Aplicación

Immunofluorescence, cytotoxicity, immunoprecipitation

Medio de cultivo

RPMI 1640 + 2mM glutamine + 10% FBS

Rutina de subcultivo

Maintain cultures between 3-9 x 100,000 cells/ml; 5% CO₂; 37°C

Otras notas

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