3T3-MSV

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA.

• Código UNSPSC: 41106514

Propiedades

• origen biológico: mouse embryo
• envase: tube of 5 μg 90092701-DNA-5UG; pkg of vial of cells 90092701-1VL
• modo de crecimiento: Adherent
• cariotipo: Not specified
• morfología: Fibroblast
• productos: Growth factor
• receptores: Not specified
• técnicas: cell culture | mammalian: suitable
• enfermedades relevantes: cancer
• Condiciones de envío: dry ice
• temp. de almacenamiento: −196°C

Descripción

Descripción general

Product has lost contact inhibition

Origen línea celular

Mouse Swiss 3T3 embryo, Moloney-MSV transformed

Descripción línea celular

This cell line is of Swiss 3T3 origin, transformed by the Moloney murine sarcoma virus. It is a non-producer of the virus particles. In serum-free media the line will release a growth factor which can be used to stimulate quiescent cells to grow in monolayer as well as in soft agar.

Aplicación

Cell growth studies

Medio de cultivo

DMEM + 2mM Glutamine + 5% Foetal Bovine Serum (FBS).

Rutina de subcultivo

Split sub-confluent cultures (70-80%) 1:6 to 1:8 i.e. seeding at 1-2x10,000 cells/cm² using 0.25% trypsin; 5% CO₂; 37°C. Care is required during subculture as cells have a tendency to easily detach from the tissue culture flask.

Otras notas

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