Clone C6/36

89051705, insect larvae, Not specified

• UNSPSC Code: 41106514

Propiedades

• product name: Clone C6/36, 89051705
• biological source: insect larvae
• growth mode: Adherent
• karyotype: Diploid
• morphology: Not specified
• products: Not specified
• receptors: Not specified
• technique(s): cell culture | mammalian: suitable
• shipped in: dry ice
• storage temp.: −196°C

Descripción

Cell Line Origin

Mosquito larvae, Aedes albopictus

Cell Line Description

Derived from Aedes albopictus cells. Useful for propagation of Dengue virus and flaviviruses. Cells are non-anchorage dependant, not tumourigenic and maintain a diploid chromosome number.

Application

Clone C6/36 has been used to screen anti-Wolbachia compounds. It has also been used to study the effects of tick-borne encephalitis virus (TBEV) vaccine.

Culture Medium

EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS) (Sodium Bicarbonate (NaHCO₃) level of 1.1g/l is recommended).

Subculture Routine

Split sub-confluent cultures (70-80%) 1:4 to 1:10 i.e. seeding at 5000 - 30000 cells/cm² . Cells are dislodged by mechanical agitation; 5% CO₂; 28°C. Freeze in 10% glycerol.

Other Notes

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