H139-52

Reactivity: Anti-κ-Chain, mouse, 85103111

• UNSPSC Code: 12352200

Propiedades

• biological source: Mouse x Rat Heterohybridoma
• ImmunoDon: LOU/Ws1 rat spleen
• Myeloma: P3X63Ag8.653
• Reactivity: Anti-κ-Chain, mouse
• technique(s): cell culture | mammalian: suitable
• shipped in: dry ice
• storage temp.: −196°C

Descripción

Cell Line Origin

Mouse P3X63Ag8.653 x Rat LOU/Ws1 spleen

Cell Line Description

The antibody is directed against murine kappa chain.

Immunogen

Purified murine immunoglobulin

Application

The isotype of the monoclonal antibody produced by this cell line is rat IgG1, kappa.

Culture Medium

DMEM + 2mM glutamine + 10% Foetal Bovine Serum (FBS)

Subculture Routine

Maintain cultures between 3-9x100,000 cells/ml; 5% CO₂; 37°C. These cells require mouse macrophage feeder layers upon resuscitation

Other Notes

Cultures from HPA Culture Collections and supplied by Sigma are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.