AFRC MAC 10

Reactivity: Anti-Glycoprotein of Chlamydomonas reinhardii, 85102274, NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA.

• Código UNSPSC: 41106514

Propiedades

• origen biológico: Rat x Rat Hybridoma
• envase: tube of 5 μg 85102274-DNA-5UG; pkg of vial of cells 85102274-1VL
• ImmunoDon: AO rat spleen
• Mieloma: YB2/3.0 Ag20
• Reactividad: Anti-Glycoprotein of Chlamydomonas reinhardii
• técnicas: cell culture | mammalian: suitable
• isotipo: IgM
• Condiciones de envío: dry ice
• temp. de almacenamiento: −196°C

Descripción

Origen línea celular

Rat YB2/3.0 Ag20 x Rat AO spleen

Descripción línea celular

The antibody recognises the glycoprotein 2BII. Binding is lost after acid hydrolysis or de-glycosylation of 2BII. AFRC MAC 10 antibody shows similar fine specificity and properties to AFRC MAC 6, 7, 8, 9 and 11.

Inmunógeno

2BII, the major glycoprotein fraction of the cell wall of Chlamydomonas reinhardii

Medio de cultivo

DMEM + 2mM glutamine + 10% Foetal Bovine Serum (FBS)

Rutina de subcultivo

Maintain cultures between 3-9x100,000 cells/ml; 5% CO₂; 37°C. These cells require mouse macrophage feeder layers upon resuscitation

Otras notas

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