C8868

Cholesterol Oxidase from Brevibacterium

lyophilized powder, ≥50 units/mg protein, recombinant, expressed in E. coli

• Sinónimos: Cholesterol: oxygen oxidoreductase
• Número CAS: 9028-76-6
• UNSPSC Code: 12352204
• NACRES: NA.54
• EC Number: 232-842-1
• MDL number: MFCD00130783
• Número CE: 1.1.3.6 (BRENDA, IUBMB)
• Specific activity: ≥50 units/mg protein
• Recombinant: expressed in E. coli

Propiedades

• recombinant: expressed in E. coli
• form: lyophilized powder
• specific activity: ≥50 units/mg protein
• mol wt: 64 kDa
• composition: Protein, ≥15% biuret
• storage temp.: −20°C

Descripción

General description

Type II cholesterol oxidase containing FAD cofactor covalently linked to the enzyme.

Application

Cholesterol oxidase has been used in a study to demonstrate that microheterogeneity in hydrophobic-hydrophilic block copolymer systems can be exploited for immobilizing enzymes and to carry out enzymatic reactions. Cholesterol oxidase has also been used in a study that concluded that amphipaths that activate cholesterol might be useful in treating NPC disease.

Cholesterol oxidase is used to determine serum cholesterol.[1][2] The enzyme also finds application in the microanalysis of steroids in food samples and in distinguishing 3-ketosteroids from 3β-hydroxysteroids.[3] Transgenic plants expressing cholesterol oxidase are being investigated in the fight against the cotton boll weevil.[4] CHOD has also been used as a molecular probe to elucidate cellular membrane structures.[5][6]

Biochem/physiol Actions

Cholesterol oxidase (CHOD) is a monomeric flavoprotein containing FAD that catalyzes the first step in cholesterol catabolism. This bifunctional enzyme oxidizes cholesterol to cholest-5-en-3-one in an FAD-requiring step, which is then isomerized to cholest-4-en-3-one with the release of H₂O₂.[7]

Physical form

Supplied as a lyophilzed powder containing sucrose

Other Notes

One unit will convert 1.0 μmole of cholesterol to 4-cholesten-3-one per min at pH 7.5 at 25 °C. Note: 4-cholesten-3-one may undergo isomerization.

Información de seguridad

• pictograms: GHS08
• signalword: Danger
• hcodes: H334
• pcodes: P261 - P284 - P501
• Hazard Classifications: Resp. Sens. 1
• Clase de almacenamiento: 11 - Combustible Solids
• wgk: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves, type N95 (US)