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MilliporeSigma

C8696

Sigma-Aldrich

Cathepsin D from human liver

lyophilized powder, ≥250 units/mg protein (E1%/280)

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About This Item

Número de CAS:
Comisión internacional de enzimas:
Número MDL:
Código UNSPSC:
12352204
NACRES:
NA.32

Formulario

lyophilized powder

Nivel de calidad

actividad específica

≥250 units/mg protein (E1%/280)

mol peso

~45 kDa

color

white

Nº de acceso UniProt

temp. de almacenamiento

−20°C

Información sobre el gen

human ... CTSD(1509)

Descripción general

Cathepsin D is an aspartic protease, which is located in lysosomes. It is involved in protein catabolism and maintains hormone and antigen processing. Cathepsin D is implicated in neoplasia and neurodegenerative changes. It regulates lysosomal proteolysis and endogenous fibrinolysis.

Aplicación

Cathepsin D from human liver has been used:
  • in β-secretase activity assay
  • for enzymatic degradation of amyloid β 1-42
  • in microinjection of human foreskin fibroblasts

Acciones bioquímicas o fisiológicas

Cathepsin D is an endosomal-lysosomal aspartic protease implicated in breast cancer metastasis and Alzheimer′s disease. Lysosomal release of cathepsin D has been found to precede cytochrome c release and loss of membrane potential in apoptotic human foreskin fibroblasts. Cathepsin D levels in PC12 cells increase 12 to 24 hours after apoptosis is induced.
Endosomal-lysosomal aspartic protease implicated in breast cancer metastasis and Alzheimer′s disease. Lysosomal release of cathepsin D has been found to precede cytochrome c release and loss of membrane potential in apoptotic human foreskin fibroblasts.

Otras notas

Contains α, β and γ isoenzymes as observed by isoelectric-focusing.

Definición de unidad

One unit will produce an increase in A280 of 1.0 in 30 min at pH 3.3 at 37 °C measured as TCA-soluble products using acid-denatured hemoglobin as substrate (1 cm light path).

Forma física

Powder containing sodium phosphate buffer salt.

Inhibidor

Referencia del producto
Descripción
Precios

Producto relacionado

Código de clase de almacenamiento

11 - Combustible Solids

Clase de riesgo para el agua (WGK)

WGK 3

Punto de inflamabilidad (°F)

Not applicable

Punto de inflamabilidad (°C)

Not applicable


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Visite la Librería de documentos

Platelet membrane beta-secretase activity in mild cognitive impairment and conversion to dementia: a longitudinal study
McGuinness B, et al.
Journal of Alzheimer'S Disease, 49(4), 1095-1103 (2016)
Jeane do Nascimento Moraes et al.
The journal of venomous animals and toxins including tropical diseases, 28, e20220002-e20220002 (2022-11-22)
Cathepsin D (CatD) is a lysosomal proteolytic enzyme expressed in almost all tissues and organs. This protease is a multifunctional enzyme responsible for essential biological processes such as cell cycle regulation, differentiation, migration, tissue remodeling, neuronal growth, ovulation, and apoptosis.
Aspartic Proteinases Physiology and Pathology (1995)
Andrea Gilg et al.
Journal of translational medicine, 19(1), 190-190 (2021-05-05)
Endogenous Peptide Inhibitor of CXCR4 (EPI-X4) is a natural antagonist of the CXC chemokine receptor 4 (CXCR4). EPI-X4 is a 16-mer peptide that is released from human serum albumin (HSA) by acidic aspartic proteases such as Cathepsin D and E.
Microinjection of cathepsin d induces caspase-dependent apoptosis in fibroblasts
Roberg K, et al.
The American Journal of Pathology, 161(1), 89-96 (2002)

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