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C6158

Crystal Violet

≥90% purity anhydrous basis, ACS reagent, powder

Sinónimos:

Basic Violet 3, Gentian Violet, Hexamethylpararosaniline chloride, Methyl Violet 10B

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Talla/SKUDisponibilidadPrecio
50 g
Comprobar disponibilidad del carrito
$81.70
100 g
Comprobar disponibilidad del carrito
$157.00

Acerca de este artículo

Fórmula lineal:
C25H30N3Cl
Número CAS:
548-62-9
Peso molecular:
407.98
UNSPSC Code:
12171500
NACRES:
NA.47
PubChem Substance ID:
57654029
EC Number:
208-953-6
MDL number:
MFCD00011750
Beilstein/REAXYS Number:
3580948
Colour Index Number:
42555

$81.70

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Nombre del producto

Crystal Violet, ACS reagent, ≥90.0% anhydrous basis

grade

ACS reagent

Quality Segment

assay

≥90.0% anhydrous basis

form

powder

loss

≤7.5% loss on drying

color

green to very dark green

pH

2.5-3.5 (20 °C, 10 g/L)

mp

205 °C (dec.) (lit.)

solubility

water: soluble 50 g/L at 27 °C

density

1.19 g/cm3 at 20 °C

absorption

passes test

suitability

passes test for sensitivity as indicator

antibiotic activity spectrum

fungi

application(s)

diagnostic assay manufacturing
hematology
histology

mode of action

cell membrane | interferes, enzyme | inhibits

storage temp.

room temp

SMILES string

[Cl-].CN(C)c1ccc(cc1)\C(c2ccc(cc2)N(C)C)=C3/C=C\C(C=C3)=[N+](/C)C

InChI

1S/C25H30N3.ClH/c1-26(2)22-13-7-19(8-14-22)25(20-9-15-23(16-10-20)27(3)4)21-11-17-24(18-12-21)28(5)6;/h7-18H,1-6H3;1H/q+1;/p-1

InChI key

ZXJXZNDDNMQXFV-UHFFFAOYSA-M

Application

Crystal violet has been used to determine cell viability. It has been used in the staining of cells for the quantification of cellular migration.

Biochem/physiol Actions

Crystal violet can be used for DNA visualization in agarose gels. The dye is used only in the presence of high concentrations of DNA. Crystal violet is also used for the staining of bacteria in gram staining technique. It is also used for the staining of plant chromosomes. Crystal violet helps in colorimetric measurement of cell viability.

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Este artículo
ATE638638214C077546364
Crystal Violet ACS reagent, ≥90.0% anhydrous basis

Sigma-Aldrich

C6158

Crystal Violet

CRYSTAL VIOLET

AstaTech, Inc.

ATE638638214

CRYSTAL VIOLET

Crystal Violet certified by the BSC

Sigma-Aldrich

C0775

Crystal Violet

Crystal Violet VETRANAL®, analytical standard

Supelco

46364

Crystal Violet

description

ACS reagent, ≥90.0% anhydrous basis

description

-

description

certified by the BSC

description

VETRANAL®, analytical standard

grade

ACS reagent

grade

-

grade

-

grade

analytical standard

suitability

passes test for sensitivity as indicator

suitability

-

suitability

-

suitability

-

color

green to very dark green

color

-

color

green to very dark green

color

-

application(s)

diagnostic assay manufacturing
hematology
histology

application(s)

-

application(s)

diagnostic assay manufacturing
hematology
histology

application(s)

cleaning products
cosmetics
environmental
food and beverages
personal care

form

powder

form

-

form

powder

form

-

solubility

water: soluble 50 g/L at 27 °C

solubility

-

solubility

H2O: 1 mg/mL

solubility

water: soluble 50 g/L at 27 °C

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Palabra de advertencia

Danger

Códigos de peligro

H302,H318,H351,H410

Hazard Classifications

Acute Tox. 4 Oral - Aquatic Acute 1 - Aquatic Chronic 1 - Carc. 2 - Eye Dam. 1

Clase de almacenamiento

11 - Combustible Solids

Punto de inflamación (°F)

Not applicable

Punto de inflamación (°C)

Not applicable

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Questions

1–2 of 2 Questions  

  1. How to prepare a crystal violet solution for plaque titration assay with this reference C6158-50G sigma?

    1 answer

    1. Would suggest adding 0.5 grams of Crystal Violet to 100 ml water. Mix well to dissolve. Then add 20 ml methanol. IF this solution results in overstaining of the plaques, prepare a fresh solution with a lower percentage of Crystal Violet

      A published procedure for plaque assay titration is not available. The methods listed in the literature vary widely from source to source. Some procedures will fix the plaques first with either methanol or acetone. Other procedures will dilute the Crsytal Violet at various percentages. The percentages can vary from 0.5% to 0.001% depending upon the source. The various sources typically state the crystal violet solution should either be prepared fresh or stored no longer than 1-2 weeks at 4°C.

      Helpful?

  2. can we make the solution just by dissolving in water?

    1 answer

    1. Yes, this product is soluble in water at 50 mg/mL at 20 °C.

      Helpful?

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