C2979

Caspase 7 human

≥95% (SDS-PAGE), recombinant, expressed in E. coli, buffered aqueous solution, ≥1,500 units/mg protein

• Sinónimos: CMH-1, ICE-LAP3, Mch-3
• Número MDL: MFCD01865995
• Código UNSPSC: 12352200
• NACRES: NA.32

Propiedades

• recombinante: expressed in E. coli
• Ensayo: ≥95% (SDS-PAGE)
• Formulario: buffered aqueous solution
• actividad específica: ≥1,500 units/mg protein
• mol peso: 56 kDa by immunoblotting
• Nº de acceso UniProt: P55210
• Condiciones de envío: dry ice
• temp. de almacenamiento: −70°C
• Información sobre el gen: human ... CASP7(840)

Descripción

Acciones bioquímicas o fisiológicas

Cleaves the substrates consisting of consensus sequence DEVD.

Member of the CED-3 subfamily, the effector caspases, with an essential role in the apoptotic process. Upon activation, the zymogen is translocated to the mitochondrial and microsomal fractions. It is responsible for the cleavage of cellular substrates that precipitate the morphological changes of apoptosis.

Member of the CED-3 subfamily, the effector caspases; upon activation, the zymogen is translocated to the mitochondrial and microsomal fractions.

Useful in screening caspase inhibitors, studying enzyme regulation, determining specificities of caspase substrates, as well as using as a positive control in caspase activity assays and Western blot analyses.

Definición de unidad

One unit will hydrolyze 1.0 nmol of Ac-Asp-Glu-Val-Asp-AFC per minute at pH 7.5 at 25 °C.

Forma física

Solution in HEPES, NaCl, DTT and sucrose, without carrier protein

Nota de análisis

Migrates as two major polypeptides (20 kDa and 10 kDa) in immunoblotting. Two minor polypeptides derived from the large subunit are also present.

Información de seguridad

• Código de clase de almacenamiento: 12 - Non Combustible Liquids
• Clase de riesgo para el agua (WGK): WGK 2
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable
• Equipo de protección personal: Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)