C2522

Carbonic Anhydrase Isozyme II from bovine erythrocytes

lyophilized powder, ≥3,000 W-A units/mg protein

• Sinónimos: Carbonate Dehydratase, Carbonate Hydrolyase, Carbonic Anhydrase II
• Número CAS: 9001-03-0
• UNSPSC Code: 12352204
• NACRES: NA.54
• EC Number: 232-576-6
• MDL number: MFCD00081467
• Número CE: 4.2.1.1 (BRENDA, IUBMB)
• Specific activity: ≥2475 units/mg solid, ≥3,000 W-A units/mg protein
• Biological source: bovine erythrocytes

Propiedades

• biological source: bovine erythrocytes
• Quality Segment: 300
• form: lyophilized powder
• specific activity: ≥2475 units/mg solid, ≥3,000 W-A units/mg protein
• mol wt: 30 kDa
• pI: ~5.9
• storage temp.: −20°C

Descripción

Application

Carbonic anhydrase from sigma has been used for the analysis of thermodynamic stability of the enzyme by measuring the binding affinity and stoichiometry. The enzyme has also been used to generate CD₄⁺ T cell lines specific for carbonic anhydrase during the study of autoimmune pancreatitis in rat.

Biochem/physiol Actions

Carbonic anhydrase is a zinc metalloenzyme that has a molecular weight of approximately 30,000 Da. The enzyme catalyzes the hydration of carbon dioxide to carbonic acid. It is involved in many physiological and pathological processes such as pH and CO₂ homeostasis, transport of bicarbonate and CO₂, biosynthetic reactions, bone resorption, calcification, and tumorigenicity. Therefore, inhibitors that block this enzyme can have therapeutic implications in glaucoma, epilepsy and Parkinson′s disease.

Other Notes

One Wilbur-Anderson (W-A) unit will cause the pH of a 0.02 M Trizma buffer to drop from 8.3 to 6.3 per min at 0 °C. (One W-A unit is essentially equivalent to one Roughton-Booth unit.)

Información de seguridad

• Pictogramas: GHS08
• Palabra de advertencia: Danger
• Códigos de peligro: H334
• Indicaciones de precaución: P261 - P284 - P501
• Hazard Classifications: Resp. Sens. 1
• Clase de almacenamiento: 11 - Combustible Solids
• ¿Qué está pasando?: WGK 3
• Punto de inflamación (°F): Not applicable
• Punto de inflamación (°C): Not applicable
• EPI (equipos de protección individual): Eyeshields, Gloves, type N95 (US)