C2249

Human Collagen Type I

from human fibroblasts, liquid, 3 mg/mL, suitable for cell culture

• Sinónimos: Collagen Solution, Fibroblast Collagen
• UNSPSC Code: 12352202
• NACRES: NA.75

Propiedades

• Nombre del producto: Collagen Solution from human fibroblasts, 3 mg/mL, sterile-filtered, BioReagent, suitable for cell culture
• biological source: human fibroblasts
• Quality Level: 100
• sterility: sterile-filtered
• product line: BioReagent
• form: solution
• packaging: pkg of 20 mL
• concentration: 3 mg/mL
• technique(s): cell culture | mammalian: suitable
• surface coverage: 6‑10 μg/cm²
• binding specificity: Peptide Source: Collagen; ; Peptide Source: Elastin
• foreign activity: endotoxin ≤5.0 μmole/min-mg protein
• shipped in: wet ice
• storage temp.: 2-8°C

Descripción

General description

Collagen constitutes to one-third of the total protein in humans.[1] Collagen fibril is the basic component of the tissues containing collagen. It is predominantly expressed in the extracellular matrix.[1]Type I collagen is a major structural component of skin, bone, tendon, and other fibrous connective tissues. It differs from other collagens by its low lysine hydroxylation and low carbohydrate composition. Type I collagen is a heterotrimer composed of two α1(I) chains and one alpha2(I) chain, which spontaneously form a triple helix scaffold at neutral pH and 37 °C. Purified human collagen solution is produced from neo-natal human fibroblasts that have secreted human extracellular matrix (hECM) using a propriety cell culture production system. This product contains a high monomer content. In vitro cultures were prepared using intensively tested human fibroblast cells and purified using a manufacturing process following applicable aspects of cGMP. The human fibroblast cells have undergone significant testing demonstrating safety from viruses and other adventitious agents. This process contains built-in, validated steps to ensure the inactivation of possible prion and/or viral contaminants.

Application

Collagen Solution from human fibroblasts has been used

• as a component of coating solution for human bone marrow chip fabrication.[2]
• in fibroblast-like synoviocytes culturing.[3]
• as a coating solution for microchannels for organ chip culture[4]

Biochem/physiol Actions

Collagen is an essential ingredient of connective tissue. Studies in a Chinese family show that mutation in COL1A1 (collagenase type I) is linked with type I osteogenesis imperfecta.[5][6] Collagen is linked with subchondral turnover of bone, and might have potential as marker to determine the state of joint space narrowing and osteophytes in osteoarthritis.[7]

Proteins belonging to the collagen family supports and nourishes body tissues such as tendons, cartilage, skin and sclera of the eye.[8] Collagen type 1 is a versatile building material required for tissue elasticity and maintains its stability and strength.[9] Collagen is also present in byssus thread of invertebrates, which helps in surface attachment.[9] Defective collagen affects the biomechanical property of arterial tissues.[10]

Información de seguridad

• Storage Class: 10 - Combustible liquids
• wgk_germany: nwg
• flash_point_f: Not applicable
• flash_point_c: Not applicable