C1999
CMP-Sialic Acid Synthetase from Neisseria meningitidis group B
recombinant, expressed in E. coli BL21, ≥10 units/mg protein
Sinónimos:
CTP: N-Acylneuraminate cytidylyltransferase
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| Talla/SKU | Disponibilidad | Precio |
|---|---|---|
10 units | Comprobar disponibilidad del carrito | $372.00 |
Acerca de este artículo
Specific activity:
≥10 units/mg protein
Recombinant:
expressed in E. coli BL21
$372.00
Comprobar disponibilidad del carrito
Servicio técnico
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Permítanos ayudarlerecombinant
expressed in E. coli BL21
Quality Segment
form
lyophilized solid
specific activity
≥10 units/mg protein
mol wt
26.0 kDa
shipped in
dry ice
storage temp.
−20°C
General description
Cytidine monophosphate (CMP)-Sialic Acid Synthetase from Neisseria meningitidis group B is encoded in neuA gene. The protein has a molecular weight of 24.8 kDa.
Biochem/physiol Actions
Cytidine monophosphate (CMP)-sialic acid synthetase catalyses the conversion of N?acetylneuraminic acid (NeuNAc) to CMP-NeuNAc. CMP-sialic acid synthetase has globular α/β domain and is categorised under αβα three-layered sandwich fold. The dimerization domain aids the interaction between the monomers. It also has mononucleotide binding and NeuAc binding pocket. Mg2+ is essential for the catalytic functionality of CMP-sialic acid synthetase.
Physical form
Supplied as a lyophilized powder containing Tris-HCl and NaCl.
Analysis Note
Enzymatic activity assays are performed in Tris-HCl buffer (100 mM, pH 8.5) containing Neu-5-Ac (1 mM) and CTP (1 mM) at 37 °C for 30 min and analyzed using capillary electrophoresis with a UV detector (200 nm).
Other Notes
One unit will catalyze the formation of 1 μmol CMP-Neu-5-Ac from Neu-5-Ac and CTP per minute at 37 °C at pH 8.0.
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Este artículo | ||
|---|---|---|
| description recombinant, expressed in E. coli BL21, ≥10 units/mg protein | description CMP-sialic acid is the activated form of sialic acid that is required for the biosynthesis of sialic acid-containing complex carbohydrates. | description ≥85% (HPLC) |
| specific activity ≥10 units/mg protein | specific activity - | specific activity - |
| recombinant expressed in E. coli BL21 | recombinant - | recombinant - |
| form lyophilized solid | form solid | form powder |
| mol wt 26.0 kDa | mol wt - | mol wt - |
| storage temp. −20°C | storage temp. −20°C | storage temp. −20°C |
| shipped in dry ice | shipped in wet ice | shipped in - |
Clase de almacenamiento
11 - Combustible Solids
¿Qué está pasando?
WGK 3
Punto de inflamación (°F)
Not applicable
Punto de inflamación (°C)
Not applicable
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