C-12810

Human Cardiac Myocytes (HCM)

500,000 cryopreserved cells

• Sinónimos: HCM cells
• NACRES: NA.81
• UNSPSC Code: 41106514
• Biological source: human heart (ventricles)
• Morphology: (myocytes)

Propiedades

• biological source: human heart (ventricles)
• product line: PromoCell^®
• packaging: pkg of 500,000 cells
• morphology: (myocytes)
• technique(s): cell culture | mammalian: suitable
• shipped in: dry ice
• storage temp.: −196°C

Descripción

General description

Lot specific orders are not able to be placed through the web. Contact your local sales rep for more details.
Our HCM are now also available from HLA-typed and Diabetes Type I and Type II/Cardiomypathy donors.

Application

Primary Human Cardiac Myocytes (HCM) are isolated from the ventricles of the adult heart and are provided in a cryopreserved format. They are qualified for in vitro research on cardiac diseases and for pharmacological studies. Unlike freshly isolated rod-shaped myocytes, cultured HCM can be used for long-term experiments like investigating the long-term effects of cytokines, mechanical strain, or cell-cell interactions, as they are prepared according to a special protocol.Initially, the HCM act more like progenitor cells in that they are not yet fully differentiated. They express the markers of early stage differentiation such as GATA-4 and sarcomeric alpha-actin and have a high capacity for proliferation. When they are grown to confluency and cultivated for an extended period of time, the differentiation process begins. Markers of late differentiation (e.g. sarcomeric alpha-actinin, slow muscle myosin) are increased and the cells begin to form myotube-like structures.

Biochem/physiol Actions

Heart

Preparation Note

Click here for more information.

Analysis Note

Rigid quality control tests are performed for each lot of Human Cardiac Myocytes. They are tested for cell morphology, adherence rate, and cell viability. Furthermore, flow cytometric analyses for cell-type specific markers, e.g. sarcomeric a-actinin and slow muscle myosin, are carried out for each lot. Growth performance is tested through multiple passages up to 15 population doublings (PD) under culture conditions without antibiotics and antimycotics. In addition, all cells have been tested for the absence of HIV-1, HIV-2, HBV, HCV, HTLV-1, HTLV-2 and microbial contaminants (fungi, bacteria, and mycoplasma).

Other Notes

Recommended Plating Density: 10000 - 15000 cells per cm²Passage After Thawing: P2Tested Markers: Sarcomeric alpha-actinin positive; Slow muscle myosin positiveGuaranteed population doublings: >15

Recommended Primary Cell Culture Media:Link

Legal Information

PromoCell is a registered trademark of PromoCell GmbH

Disclaimer

Although tested negative for HIV-1, HIV-2, HBV, HCV, HTLV-1 and HTLV-2, the cells – like all products of human origin – should be handled as potentially infectious. No test procedure can completely guarantee the absence of infectious agents.

Información de seguridad

• Clase de almacenamiento: 12 - Non Combustible Liquids
• ¿Qué está pasando?: WGK 1
• Punto de inflamación (°F): Not applicable
• Punto de inflamación (°C): Not applicable