C-12735

Human White Preadipocytes (HWP-c)

Subcutaneous, 1,000,000 cryopreserved cells

• NACRES: NA.81
• UNSPSC Code: 41106514
• Biological source: human adipose tissue (subcutaneous)

Propiedades

• biological source: human adipose tissue (subcutaneous)
• form: liquid
• packaging: pkg of 1,000,000 cells
• technique(s): cell culture | mammalian: suitable
• shipped in: dry ice
• storage temp.: −196°C

Descripción

General description

Lot specific orders are not able to be placed through the web. Contact your local sales rep for more details.
Our subcutaneous HWP are now also available from Diabetes Type I and Type II and HLA-typed donors.

Application

Primary Human White Preadipocytes (HWP) are isolated from adult subcutaneous adipose tissue from different locations (lot specific source information is available on request) and provided in a cryopreserved format. Adipose tissue is crucial in energy storage and metabolic homeostasis. An increase in adipose tissue results either from an enlargement of mature adipocytes or from the differentiation of adipocyte precursor cells (preadipocytes) into new mature adipocytes. These preadipocytes are already present in adipose tissues. They can proliferate throughout adult life and replace the cells that have differentiated into mature adipocytes. Since this differentiation process is controlled by a variety of growth factors and hormones, preadipocytes are suitable for the investigation of the physiological mechanisms controlling proliferation, differentiation, and function of adipose tissue.

Preparation Note

Click here for more information.

Analysis Note

Rigid quality control tests are performed for each lot of PromoCell Human White Preadipocytes. They are tested for cell morphology, adherence rate, and cell viability. Growth performance is tested through multiple passages up to 10 population doublings (PD) under culture conditions without antibiotics and antimycotics. Furthermore, each lot of PromoCell Human White Preadipocytes is extensively tested for its capacity to differentiate into mature adipocytes. In addition, all cells have been tested for the absence of HIV-1, HIV-2, HBV, HCV, HTLV-1, HTLV-2 and microbial contaminants (fungi, bacteria, and mycoplasma).

Other Notes

Recommended Plating Density: 5000 cells per cm²
Passage After Thawing: P2
Tested Markers: Differentiation tested
Population Doublings: >10

Recommended Adipocyte Nutrition Medium:Link

Recommended Preadipocyte Growth Medium:Link

Disclaimer

Although tested negative for HIV-1, HIV-2, HBV, HCV, HTLV-1 and HTLV-2, the cells – like all products of human origin – should be handled as potentially infectious. No test procedure can completely guarantee the absence of infectious agents.

Información de seguridad

• Clase de almacenamiento: 12 - Non Combustible Liquids
• ¿Qué está pasando?: WGK 1
• Punto de inflamación (°F): Not applicable
• Punto de inflamación (°C): Not applicable