B9139

Biotin-dextran

mol wt 10,000, Lysine-fixable

• UNSPSC Code: 12352201
• eCl@ss: 34058011
• NACRES: NA.25
• MDL number: MFCD00130657

Propiedades

• conjugate: biotin conjugate
• Quality Segment: 200
• form: powder
• mol wt: 10,000
• extent of labeling: ~2 mol per mol
• color: white
• solubility: H₂O: soluble 50 mg/mL, clear, colorless to faintly yellow
• storage temp.: 2-8°C

Descripción

Application

Biotin-dextran is used in tissue staining to prepare neurons for detection by avidin-conjugated detection systems. It is taken up by neuronal processes and transported bi-directionally. It infiltrates axon boutons in the anterograde direction and dendritic processes in the retrograde direction.[1] Staining can be observed in fixed and sectioned tissue from two days to two weeks after biotin-dextran is injected into the brain or spinal cord. It can also be applied to cut nerve tracts.[2] Biotinylated neurons are detected by either light or electron microscopy following incubation with avidin-horseradish peroxidase conjugate and the electron-dense substrate 3,3′-diaminobenzidine (DAB).[3]

Other Notes

To gain a comprehensive understanding of our extensive range of Dextrans for your research, we encourage you to visit our Carbohydrates Category page.

Información de seguridad

• Clase de almacenamiento: 11 - Combustible Solids
• wgk: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves, type N95 (US)