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B7632

Sigma-Aldrich

N-Benzoyl-Phe-Val-Arg-p-nitroanilide hydrochloride

chromogenic, protease substrate, ≥98% (TLC), powder

Sinónimos:

N-Benzoyl-L-phenylalanyl-L-valyl-L-arginine-4-nitroanilide hydrochloride

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5 MG
$244.00
10 MG
$423.00
50 MG
$1,470.00

$244.00


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5 MG
$244.00
10 MG
$423.00
50 MG
$1,470.00

About This Item

Fórmula empírica (notación de Hill):
C33H40N8O6 · HCl
Número de CAS:
Peso molecular:
681.18
Beilstein/REAXYS Number:
3027332
MDL number:
UNSPSC Code:
12352204
PubChem Substance ID:
NACRES:
NA.32

$244.00


En stockDetalles


Nombre del producto

N-Benzoyl-Phe-Val-Arg-p-nitroanilide hydrochloride, protease substrate

Quality Level

assay

≥98% (TLC)

form

powder

solubility

methanol: 20 mg/mL, clear, colorless to light yellow

storage temp.

−20°C

SMILES string

Cl.CC(C)[C@H](NC(=O)[C@H](Cc1ccccc1)NC(=O)c2ccccc2)C(=O)N[C@@H](CCCNC(N)=N)C(=O)Nc3ccc(cc3)[N+]([O-])=O

InChI

1S/C33H40N8O6.ClH/c1-21(2)28(40-31(44)27(20-22-10-5-3-6-11-22)39-29(42)23-12-7-4-8-13-23)32(45)38-26(14-9-19-36-33(34)35)30(43)37-24-15-17-25(18-16-24)41(46)47;/h3-8,10-13,15-18,21,26-28H,9,14,19-20H2,1-2H3,(H,37,43)(H,38,45)(H,39,42)(H,40,44)(H4,34,35,36);1H/t26-,27-,28-;/m0./s1

InChI key

PYVSMZDQQUZGPF-JAQKLANPSA-N

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General description

N-Benzoyl-Phe-Val-Arg-p-nitroanilide is a chromogenic protease substrate.[1]

Application

  • N

  • -Benzoyl-Phe-Val-Arg-p-nitroanilide hydrochloride has been used: as a substrate: for trypsin-like enzyme in the soluble and particulate fractions of the hyphae[2]
  • for the thrombin, recombinant and native batroxobin from snake venom[3]
  • for fibrinolytic enzyme aprE2 in amidolytic activity assay[4]

Packaging

Bottomless glass bottle. Contents are inside inserted fused cone.

Substrates

A substrate for trypsin, thrombin and reptilase.

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)


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Visite la Librería de documentos

Methodological considerations on antithrombin determination.
U Abildgaard
Acta chirurgica Scandinavica. Supplementum, 509, 97-99 (1982-01-01)
I J Mackie et al.
Thrombosis research, 28(4), 499-507 (1982-11-15)
The Factor VIII content of Factor IX concentrates was investigated by agarose gel electrophoresis which removed the interfering effects of stabilisers and proteolytic enzyme inhibitors. Factor VIII coagulant activity (FVIII C) as measured by clotting and amidolytic methods correlated well
Inhibition of human coagulation factor Xa by thrombin substrates.
C L Orthner et al.
Thrombosis research, 23(6), 533-539 (1981-09-15)
Hydrolysis of N-alpha-benzoyl-L-phenylalanyl-L-valyl-L-arginine-p-nitroanilide by human alpha thrombin in the presence of heparin.
M J Griffith et al.
Thrombosis research, 17(1-2), 83-90 (1980-01-01)
Substrates for determination of trypsin, thrombin and thrombin-like enzymes.
L Svendsen et al.
Folia haematologica (Leipzig, Germany : 1928), 98(4), 446-454 (1972-01-01)

Protocolos

Thrombin is an endolytic serine protease that selectively cleaves the Arg–Gly bonds of fibrinogen to form fibrin and release fibrinopeptides A and B.

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