B2059

Biotin Polyethyleneoxide Iodoacetamide

• Sinónimos: Biotin PEO Iodoacetamide
• Fórmula empírica (notación de Hill): C₁₈H₃₁IN₄O₅S
• Peso molecular: 542.43
• NACRES: NA.32
• PubChem Substance ID: 24891627
• UNSPSC Code: 12352119
• MDL number: MFCD04974830
• Form: powder

Propiedades

• description: Sulfhydryl specific, water soluble biotinylation reagent
• Quality Segment: 200
• form: powder
• technique(s): protein mass spectrometry: suitable
• solubility: H₂O: 10 mg/mL
• storage temp.: 2-8°C
• SMILES string: ICC(=O)NCCOCCOCCNC(=O)CCCCC1SCC2NC(=O)NC12
• InChI: 1S/C18H31IN4O5S/c19-11-16(25)21-6-8-28-10-9-27-7-5-20-15(24)4-2-1-3-14-17-13(12-29-14)22-18(26)23-17/h13-14,17H,1-12H2,(H,20,24)(H,21,25)(H2,22,23,26)
• InChI key: LWGLKVINXNMNJX-UHFFFAOYSA-N

Descripción

General description

Biotin polyethyleneoxide (PEO) iodoacetamide is a sulfhydryl (thiol) specific biotinylation reagent.

Application

• Incorporates a 12 atom hydrophilic spacer.
• Typically coupled to sulfhydryl groups at pH 7.5 - 8.5
• Especially useful for cysteine labeling in many proteomics type applications such as peptide mapping and mass spectrometry.

Biotin Polyethyleneoxide Iodoacetamide has been used as a thiol reactive reagent to demonstrate the presence of free thiols in capsid protein L1 of human papillomavirus 16. It has been used for biotinylation of proteins for ELISA (enzyme-linked immunosorbent assay) competition and pull down experiments.

Biochem/physiol Actions

Biotin polyethyleneoxide (PEO) iodoacetamide group reacts specifically with reduced thiols at pH 7.5 - 8.5. Peptides and small molecules containing thiol groups may also be labeled using this reagent. The biotin PEO iodoacetamide enables tagging of cysteine residues and conjugation to sulfhydryls in proteins making it useful in proteomics, peptide mapping, phosphopeptide analysis, and mass spectrometry.

Información de seguridad

• Pictogramas: GHS07
• Palabra de advertencia: Warning
• Códigos de peligro: H315,H319,H335
• Indicaciones de precaución: P261 - P264 - P271 - P280 - P302 + P352 - P305 + P351 + P338
• Hazard Classifications: Eye Irrit. 2 - Skin Irrit. 2 - STOT SE 3
• Órganos diana: Respiratory system
• Clase de almacenamiento: 11 - Combustible Solids
• ¿Qué está pasando?: WGK 3
• Punto de inflamación (°F): Not applicable
• Punto de inflamación (°C): Not applicable
• EPI (equipos de protección individual): dust mask type N95 (US), Eyeshields, Gloves