APOAC
Annexin V-Cy3™ Apoptosis Detection Kit
Sinónimos:
Red fluorescent Annexin V apoptosis assay
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1 KIT
$793.00
$793.00
Disponible para envío el09 de abril de 2025DesdeSAINT LOUIS
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1 KIT
$793.00
About This Item
Código UNSPSC:
12352207
NACRES:
NA.32
$793.00
Disponible para envío el09 de abril de 2025DesdeSAINT LOUIS
Productos recomendados
uso
sufficient for 200 tests
Nivel de calidad
envase
pkg of 1 kit
técnicas
flow cytometry: suitable
aplicaciones
cell analysis
detection
método de detección
fluorometric
Condiciones de envío
wet ice
temp. de almacenamiento
2-8°C
Características y beneficios
Detects apoptosis earlier in the process than DNA-based assays such as TUNEL
- Rapid labeling of cells. Cell staining takes only 10 minutes.
- No cell fixation or processing required, reducing the detection time and allowing the cells to be used for further study.
- 6-Carboxyfluorescein diacetate secondary dye is included with the kit to differentiate apoptotic cells from viable and necrotic cells.
Principio
Sigma′s Annexin V-Cy3 kit allows detection of annexin V bound to apoptotic cells by fluorescence microscopy. The Annexin V-Cy3 kit uses the dye Cy3.18 as the fluorochrome conjugated with annexin V. By microscopy, Cy3.18 fluoresces more brightly than the FITC conjugate. The kit includes the non-fluorescent compound 6-carboxyfluorescein diacetate (6-CFDA), which enters the cell and is hydrolyzed by the esterases present in living cells to the fluorescent compound 6-carboxyfluorescein, indicating that the cells are viable. This combination allows the differentiation among early apoptotic cells (annexin V positive, 6-CFDA positive), necrotic cells (annexin V positive, 6-CFDA negative), and viable cells (annexin V negative, 6-CFDA positive).
Información legal
Cy3 is a trademark of Cytiva
Solo componentes del kit
Referencia del producto
Descripción
- Annexin V Cy3.18 Conjugate from human placenta, buffered aqueous solution 10 μg
Los componentes del kit también están disponibles por separado
Referencia del producto
Descripción
SDS
- C50416-Carboxyfluorescein diacetate 10 mgSDS
Código de clase de almacenamiento
10 - Combustible liquids
Punto de inflamabilidad (°F)
Not applicable
Punto de inflamabilidad (°C)
Not applicable
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Artículos
Cellular apoptosis assays to detect programmed cell death using Annexin V, Caspase and TUNEL DNA fragmentation assays.
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