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A9378

L-Amino Acid Oxidase from Crotalus adamanteus

Name: <SC>L</SC>-Amino Acid Oxidase from <I>Crotalus adamanteus</I>
Brand: SIGMA

Type IV, ≥4.0 units/mg protein, aqueous suspension

Sinónimos:

L-AAO, L-Amino acid:oxygen oxidoreductase (deaminating)

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Número CAS:

9000-89-9

UNSPSC Code:

12352204

NACRES:

NA.54

EC Number:

232-564-0

MDL number:

MFCD00131454

Número CE:

1.4.3.2 (BRENDA, IUBMB)

Specific activity:

≥4.0 units/mg protein

Concentration:

≥5.0 mg/mL

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Propiedades

type

Type IV

Quality Segment

200

form

aqueous suspension

specific activity

≥4.0 units/mg protein

mol wt

~130 kDa

contains

toluene as preservative

concentration

≥5.0 mg/mL

solubility

H₂O: soluble 1.0 mg/mL, clear

storage temp.

2-8°C

Descripción

Application

L-amino acid oxidase (LAAO) is used to convert L-amino acids to their corresponding α-keto acids. L-amino acid oxidase, from Sigma, has been used in leucine aminopeptidase (LAP) activity assays. ³⁵S dimethylsulfoniopropionate (DMSP) has been synthesized chemically from ³⁵S L-methionine using LAAO from Sigma to form ³⁵S 3-methiolpropionate.

Biochem/physiol Actions

L-Amino acid oxidase is a flavoprotein with a molecular weight of 130 kDa. It consists of two different subunits of approximately 70,000 Da. Each molecule of holoenzyme has two FAD molecules. It is a glycoprotein containing about 2-5% carbohydrate, including sialic acid. Optimum pH is approximately 7.5.The enzyme may be reversibly inactivated by incubation in phosphate buffer, pH 7.5 at 38 °C. L-amino acid oxidase is involved in various metabolic pathways such as alanine and aspartate metabolism, methionine metabolism, valine, leucine and isoleucine degradation, tyrosine metabolism, phenylalanine metabolism, tryptophan metabolism, phenylalanine, tyrosine and tryptophan biosynthesis, and alkaloid biosynthesis. It occurs in many snake venoms apart from microorganisms and animal tissue, especially in kidney and liver.

Packaging

Package size based on protein content

Preparation Note

Dissolves in water at 1 mg/mL concentration to form a clear solution.

Other Notes

One Unit oxidizes one micromole of L-leucine per minute at 25 °C, pH 7.6

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Este artículo	A5147	A5222
Sigma-Aldrich A9378 L-Amino Acid Oxidase from Crotalus adamanteus	Sigma-Aldrich A5147 L-Amino Acid Oxidase from Crotalus atrox (Western Diamondback Rattlesnake)	Sigma-Aldrich A5222 D-Amino Acid Oxidase from porcine kidney
description Type IV, ≥4.0 units/mg protein, aqueous suspension	description Type VI, dried venom	description powder, ≥1.5 units/mg solid
specific activity ≥4.0 units/mg protein	specific activity -	specific activity ≥1.5 units/mg solid
concentration ≥5.0 mg/mL	concentration -	concentration -
form aqueous suspension	form dried venom	form powder
mol wt ~130 kDa	mol wt -	mol wt -
solubility H₂O: soluble 1.0 mg/mL, clear	solubility -	solubility -
storage temp. 2-8°C	storage temp. −20°C	storage temp. −20°C