This product is horseradish peroxidase conjugated.
A7058
Anti-polihistidina monoclonal
clone HIS-1, purified from hybridoma cell culture
Sinónimos:
Monoclonal etiqueta 6xHis, Monoclonal etiqueta His, Monoclonal etiqueta His6, Monoclonal etiqueta de epítopo 6 H, Monoclonal etiqueta de epítopo HHHHHH, Monoclonal etiqueta hexa His, Monoclonal etiqueta poli-His, Monoclonal etiquetado con histidina
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| Talla/SKU | Disponibilidad | Precio |
|---|---|---|
1 vial | Disponible para enviar HOYdesdeMILWAUKEE | $1,200.00 |
Acerca de este artículo
NACRES:
NA.56
UNSPSC Code:
12352203
Conjugate:
peroxidase conjugate
Clone:
HIS-1, monoclonal
Application:
WB
Citations:
152
Servicio técnico
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Permítanos ayudarlebiological source
mouse
Quality Segment
conjugate
peroxidase conjugate
antibody form
purified from hybridoma cell culture
antibody product type
primary antibodies
clone
HIS-1, monoclonal
form
lyophilized powder
packaging
vial of 0.5 mL
concentration
5-11 mg/mL
technique(s)
western blot: 1:2,000 using lysates of Escherichia coli induced to express a 6xHis tagged protein
isotype
IgG2a
storage temp.
2-8°C
target post-translational modification
unmodified
General description
The monoclonal anti-polyHistidine Peroxidase Conjugate antibody recognizes native and denatured forms of synthetic polyhistidine or polyhistidine-tagged fusion proteins. The product is reactive with fusion proteins expressed by prokaryotic pET, pRSET and pTrc expression vectors.
Immunogen
Proteína de fusión recombinante etiquetada con polihistidina.
Application
Antibody suitable for immunoblotting. Working dilution 1:20
Also suitable for dot blot assays and ELISA
Also suitable for dot blot assays and ELISA
Biochem/physiol Actions
El anticuerpo reconoce la polihistidina sintética, así como las formas reducidas, nativas o desnaturalizadas, de proteínas etiquetadas con 6 histidinas, expresadas en vectores seleccionados.
Physical form
Lyophilized from 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 0.05% MIT.
Preparation Note
Prepared by the two-step glutaraldehyde method described by Avrameas, S., et al., Scand. J. Immunol., 8, Suppl. 7, 7 (1978).
The antibody conjugate should be reconstituted with 0.5 ml deionized water.
Legal Information
This Product is covered by patent DE 19507166 and foreign equivalents exclusively licensed to QIAGEN GmbH, Qiagen Strasse 1, D-40724 Hilden, Germany.
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Este artículo | |||
|---|---|---|---|
| description clone HIS-1, purified from hybridoma cell culture | description clone HIS-1, purified from hybridoma cell culture | description clone HIS-1, purified immunoglobulin | description affinity isolated antibody, buffered aqueous solution |
| biological source mouse | biological source mouse | biological source mouse | biological source goat |
| antibody form purified from hybridoma cell culture | antibody form purified from hybridoma cell culture | antibody form purified immunoglobulin | antibody form affinity isolated antibody |
| conjugate peroxidase conjugate | conjugate alkaline phosphatase conjugate | conjugate - | conjugate peroxidase conjugate |
| clone HIS-1, monoclonal | clone HIS-1, monoclonal | clone HIS-1, monoclonal | clone polyclonal |
| technique(s) western blot: 1:2,000 using lysates of Escherichia coli induced to express a 6xHis tagged protein | technique(s) western blot: 1:2,000 using lysates of Escherichia coli induced to express a 6xHis tagged protein | technique(s) immunoblotting: 0.25-0.5 μg/mL, immunofluorescence: 4-8 μg/mL, flow cytometry: 3-6 μg/test | technique(s) western blot: 1:40,000-1:80,000 using total cell extract of HeLa cells, immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:200, direct ELISA: 1:40,000 |
| storage temp. 2-8°C | storage temp. 2-8°C | storage temp. −20°C | storage temp. −20°C |
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Warning
Códigos de peligro
Indicaciones de precaución
Hazard Classifications
Skin Sens. 1
Clase de almacenamiento
12 - Non Combustible Liquids
Punto de inflamación (°F)
Not applicable
Punto de inflamación (°C)
Not applicable
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To whom it may concern. Is the antibody Anti-his 6 tag hrseradish Peroxidase conjugated? Thank you
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