The method of preparation is considered proprietary. However, as mentioned in this properties section of the Product Detail Page, this is Azurin from Pseudomonas aeruginosa. See the links below to review a sample Certificate of Analysis and the product data sheet, respectively:
https://www.sigmaaldrich.com/certificates/sapfs/PROD/sap/certificate_pdfs/COFA/Q14/A3672-1MG0000211689.pdf
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/306/100/a3672dat.pdf
A3672
Azurin
from Pseudomonas aeruginosa, lyophilized powder
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1 MG
$788.50
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1 MG
$788.50
About This Item
Número de CAS:
Número CE:
Número MDL:
Código UNSPSC:
12352202
NACRES:
NA.61
Productos recomendados
origen biológico
Pseudomonas aeruginosa
Nivel de calidad
Formulario
lyophilized powder
composición
Protein, ≥65% Lowry
concentración
≥65.0% (Lowry)
técnicas
toxicology assay: suitable
solubilidad
water: soluble 1—1.1 mg/mL, clear, blue (light blue to blue)
Nº de acceso UniProt
temp. de almacenamiento
−20°C
Información sobre el gen
Pseudomonas aeruginosa ... AZU(878046)
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Descripción general
Research area: Apoptosis. Azurinis a periplasmic protein and is a homotetramer.[1]
Aplicación
Acciones bioquímicas o fisiológicas
Azurin acts as an electron donor for nitrite reductase in bacterial denitrification process.[1] It exhibits anticancer activity[1] as it hampers various independent signaling pathways associated with cancer progression. It binds to tumor suppressor protein p53 and induces cancer cell apoptosis or stalls cancer cell growth. Azurin disrupts angiogenesis by reducing the activity of VEGFR-2tyrosine kinase thereby inhibiting tumor growth. It has been observed to show cytotoxicity in human breast cancer cells and human melanoma cells.[5]
Azurin is a metalloprotein in the family of cupredoxins. It preferentially enters cancer cells over normal cells and induces apoptosis. Azurin has structural similarities to ephrinB2, and in fact binds the ephrin receptor tyrosine kinase EphB2 to initiate cell signaling that is involved in cancer progression. Azurin inhibits autophosphorlyation of the EphB2 tyrosine residue, interfering with upstream cell signaling and contributing to cancer cell growth inhibition.
Forma física
Lyophilized powder containing ammonium acetate buffer salts.
Código de clase de almacenamiento
11 - Combustible Solids
Clase de riesgo para el agua (WGK)
WGK 3
Punto de inflamabilidad (°F)
Not applicable
Punto de inflamabilidad (°C)
Not applicable
Equipo de protección personal
Eyeshields, Gloves, type N95 (US)
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Certificados de análisis (COA)
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Encuentre la documentación para los productos que ha comprado recientemente en la Biblioteca de documentos.
Caterina Bernini et al.
Journal of the American Chemical Society, 135(12), 4822-4833 (2013-03-06)
Many biological electron-transfer reactions involve short-lived tryptophan radicals as key reactive intermediates. While these species are difficult to investigate, the recent photogeneration of a long-lived neutral tryptophan radical in two Pseudomonas aeruginosa azurin mutants (Az48W and ReAz108W) made it possible
Meng Gao et al.
Protein science : a publication of the Protein Society, 26(12), 2334-2341 (2017-09-30)
Azurin secreted by Pseudomonas aeruginosa is an anticancer bacteriocin, which preferentially enters human cancer cells and induces apoptosis or growth inhibition. It turns out that azurin is a multi-target anticancer agent interfering in the p53 signaling pathway and the non-receptor
Koyu Fujita et al.
Journal of inorganic biochemistry, 115, 163-173 (2012-08-23)
Pseudoazurin (PAz), a well-characterized blue copper electron-transfer protein, is shown herein to be capable of mediating electron transfer to the nitrous oxide reductase (N(2)OR) from Achromobacter cycloclastes (Ac). Spectroscopic measurements demonstrate that reduced PAz is efficiently re-oxidized by a catalytic
Nadav Amdursky et al.
Proceedings of the National Academy of Sciences of the United States of America, 110(2), 507-512 (2012-12-26)
Measuring solid-state electron transport (ETp) across proteins allows studying electron transfer (ET) mechanism(s), while minimizing solvation effects on the process. ETp is, however, sensitive to any static (conformational) or dynamic (vibrational) changes in the protein. Our macroscopic measurements allow extending
Comparison of the self-chemisorption of azurin on gold and on functionalized oxide surfaces
Schnyder BK, et al.
Surface and Interface Analysis : SIA, 34(1), 40-44 (2002)
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How do i know that this is azurin i mean any confirmatory test or anything else.
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