A1902
Anti-Mouse IgG (whole molecule)−Alkaline Phosphatase antibody produced in rabbit
affinity isolated antibody, buffered aqueous glycerol solution
Sinónimos:
Rabbit Anti-Mouse IgG (whole molecule)−AP
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1 ML
$363.00
About This Item
Productos recomendados
origen biológico
rabbit
conjugado
alkaline phosphatase conjugate
forma del anticuerpo
affinity isolated antibody
tipo de anticuerpo
secondary antibodies
clon
polyclonal
Formulario
buffered aqueous glycerol solution
reactividad de especies
mouse
técnicas
direct ELISA: 1:1,000
western blot: suitable
Condiciones de envío
wet ice
temp. de almacenamiento
2-8°C
modificación del objetivo postraduccional
unmodified
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Descripción general
Mouse IgG is a plasma B cell derived antibody isotype defined by its heavy chain. IgG is the most abundant antibody isotype found in mouse serum.
Especificidad
Rabbit polyclonal anti-Mouse IgG (whole molecule)-Alkaline Phosphatase reacts with mouse IgA, IgG1, IgG2a, IgG2b, IgG3, and IgM myeloma proteins.
Aplicación
Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Enzyme-linked immunosorbent assay (1 paper)
Western Blotting (1 paper)
Enzyme-linked immunosorbent assay (1 paper)
Western Blotting (1 paper)
Rabbit anti-Mouse IgG (whole molecule)-alkaline phosphatase antibody has been used for ELISA and western blot assays.
Western blot analysis of lysates from the placental cell line 3ASubE-P-3 and U037 cells were performed using alkaline phosphatase conjugated rabbit anti- mouse IgG as the secondary at 1:2000 to recognize the mouse mom Il-8RB. Secondary was developed using bromochloroindoyl phosphate (Sigma) pH. 9.5 for 10-15 minutes.Western blot analysis of coimmunoprecipitates from human cell or rat cell vaginal extracts were performed using alkaline phosphatase conjugated rabbit anti- mouse IgG as the secondary at 1:15000 dilution.
Acciones bioquímicas o fisiológicas
IgG has an ability to cross the placental barrier. It acts as a complement activator and binds to the Fc-receptors on phagocytic cells. The level of IgG may vary with the status of disease or infection.
Forma física
Solution in 0.05 M Tris, pH 8.0, and 1 mM MgCl2 containing 1% bovine serum albumin, 50% glycerol, and 15 mM sodium azide.
Cláusula de descargo de responsabilidad
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Código de clase de almacenamiento
10 - Combustible liquids
Clase de riesgo para el agua (WGK)
WGK 3
Punto de inflamabilidad (°F)
Not applicable
Punto de inflamabilidad (°C)
Not applicable
Equipo de protección personal
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
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