A0561
ACCUSPIN™ System-Histopaque®-1077
sterile-filtered, density: 1.077 g/mL
Iniciar sesión para ver los precios por organización y contrato.
Seleccione un Tamaño
Cambiar Vistas
| A ustedes/SKU | Disponibilidad | Precio |
|---|---|---|
100 x 15 mL | Comprobar disponibilidad del carrito | $2,650.00 |
Acerca de este artículo
Servicio técnico
¿Necesita ayuda? Nuestro equipo de científicos experimentados está aquí para ayudarle.
Permítanos ayudarleQuality Segment
sterility
aseptically filled
form
solution
shelf life
Expiry date on the label.
packaging
package of 100 tubes
technique(s)
cell culture | mammalian: suitable
density
1.077 g/mL
application(s)
hematology
histology
storage temp.
2-8°C
General description
ACCUSPIN System-Histopaque-1077 products are intended for use in the isolation of lymphocytes and other mononuclear cells. The separation medium, Histopaque-1077, is a sterile-filtered, endotoxin-tested solution of polysucrose and sodium diatrizoate, adjusted to a density of 1.077 g/mL. ACCUSPIN System-HISTOPAQUE-1077 employs γ-irradiated 50 mL polypropylene centrifuge tubes specially designed with two chambers separated by a porous high-density polyethylene barrier ("frit"). Each tube is aseptically filled with 15 mL of Histopaque 1077. Each tube will separate 15-30 mL of anticoagulated blood.
Application
ACCUSPIN System-HISTOPAQUE-1077 employs centrifuge tubes specially designed with two chambers separated by a porous high-density polyethylene barrier ("frit"). The lower chamber contains HISTOPAQUE-1077 which allows the addition of anticoagulated whole blood without risk of mixing with the separation medium. On centrifugation, the whole blood descends through the frit to contact with the HISTOPAQUE-1077 below the frit, giving a clear separation of the blood components. The erythrocytes aggregate and the granulocytes become slightly hypertonic, increasing their sedimentation rate, resulting in pelleting at the bottom of the ACCUSPIN tube. Lymphocytes and other mononuclear cells, i.e., monocytes, remain at the plasma-HISTOPAQUE-1077 interface.
ACCUSPIN System-Histopaque-1077 is used for the separation of lymphocytes and other mononuclear cells from whole blood and bone marrow using density gradient separation media. Histopaque-1077 has been used for:
- human lymphocyte antigen (HLA) typing
- initial isolation step prior to the enumeration of T, B, and ‘null’ lymphocytes.
- preparation of pure lymphocyte suspensions for cell culture and cytotoxicity assays.
- isolation and purification of peripheral blood mononuclear cells (PBMCs) and bone marrow mononuclear cells (BMMCs)citation
- isolation of human lymphocytescitation
- isolation of breast cancer circulating tumor cells (CTCs) from the whole bloodcitation
Biochem/physiol Actions
The lower chamber contains HISTOPAQUE-1077 which allows the addition of anticoagulated whole blood without risk of mixing with the separation medium. On centrifugation, the whole blood descends through the frit to contact with the HISTOPAQUE-1077 below the frit, giving a clear separation of the blood components. The erythrocytes aggregate and the granulocytes become slightly hypertonic, increasing their sedimentation rate, resulting in pelleting at the bottom of the ACCUSPIN tube. Lymphocytes and other mononuclear cells, i.e., monocytes, remain at the plasma-HISTOPAQUE-1077 interface.
Features and Benefits
ACCUSPIN™ System-Histopaque®-1077 offers numerous advantages, such as:
- Optimal recovery of viable cells
- Permits selective separation of blood cell types
- Prevents cell distortion
- Preserves cell viability
- Minimal extraneous cell interference
- Reproducible performance from batch to batch
- Histopaque is sterile-filtered and endotoxin-tested
- Stable at 2-8°C for at least 2 years when protected from light
Legal Information
Accuspin is a trademark of Sigma-Aldrich Co. LLC
Histopaque is a registered trademark of Merck KGaA, Darmstadt, Germany
1 of 1
Este artículo | |||
|---|---|---|---|
| technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable | technique(s) - |
| form solution | form solution | form solution | form tubes |
| density 1.077 g/mL | density 1.077 g/mL | density 1.077 g/mL | density - |
| sterility aseptically filled | sterility aseptically filled | sterility aseptically filled | sterility sterile; irradiated |
| Quality Level 400 | Quality Level 400 | Quality Level 400 | Quality Level 200 |
| storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 15-25°C |
Still not finding the right product?
Explore all of our products under ACCUSPIN™ System-Histopaque®-1077
signalword
Danger
hcodes
Hazard Classifications
Resp. Sens. 1 - Skin Sens. 1
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Faceshields, Gloves, type ABEK (EN14387) respirator filter
Clase de almacenamiento
10 - Combustible liquids
Elija entre una de las versiones más recientes:
¿Ya tiene este producto?
Encuentre la documentación para los productos que ha comprado recientemente en la Biblioteca de documentos.