88417

MTT Formazan

MTT reduction end product, powder

• Sinónimos: Thiazolyl blue formazan
• Fórmula empírica (notación de Hill): C₁₈H₁₇N₅S
• Número CAS: 57360-69-7
• Peso molecular: 335.43
• UNSPSC Code: 12352204
• PubChem Substance ID: 329769650
• NACRES: NA.83
• MDL number: MFCD00080731

Propiedades

• Nombre del producto: MTT Formazan, MTT reduction end product
• Quality Segment: 100
• form: powder
• solubility: chloroform: 10 mg/mL, clear, dark violet
• SMILES string: Cc1nc(\N=N\C(=N\Nc2ccccc2)c3ccccc3)sc1C
• InChI: 1S/C18H17N5S/c1-13-14(2)24-18(19-13)23-22-17(15-9-5-3-6-10-15)21-20-16-11-7-4-8-12-16/h3-12,20H,1-2H3/b21-17+,23-22+
• InChI key: GIFKVQOPAJDJLO-SZVLODPRSA-N

Descripción

Application

MTT Formazan has been used in cell viability assay.

Biochem/physiol Actions

MTT assay is a colorimetric reaction, generally used to examine cell viability. It involves the enzymatic reduction of a yellow-colored, water-soluble, 3-[4,5-dimethylthiazole-2-yl]-2,5-diphenyltetrazolium bromide (MTT) to purple-colored, water-insoluble, MTT-formazan. This reaction is catalyzed by mitochondrial dehydrogenase and only occurs in viable cells. Thus, the amount of blue formazan is proportional to the number of viable cells. The absorbance of the formazan solution is analyzed spectrophotometrically at 570 nm.

Other Notes

Reviews: Tetrazolium salts and formazans

Información de seguridad

• Clase de almacenamiento: 11 - Combustible Solids
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves, type N95 (US)