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87918

Sigma-Aldrich

Tetramethylrhodamine isothiocyanate mixed isomers

suitable for fluorescence, mixture of isomers

Sinónimos:

MRITC, TRITC, Tetramethylrhodamine B isothiocyanate

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About This Item

Fórmula empírica (notación de Hill):
C25H21N3O3S
Número de CAS:
95197-95-8
Peso molecular:
443.52
UNSPSC Code:
12352116
NACRES:
NA.32

form

powder

solubility

DMSO: soluble
methanol: soluble

fluorescence

λex 529 nm; λem 596 nm in DMSO

suitability

suitable for fluorescence

storage temp.

2-8°C

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General description

Tetramethylrhodamine isothiocyanate, also known as TRITC is a fluorescent antibody labelling agent. It is a red-fluorescing marker and can be tracked through fluorescence microscopy.

TRITC dissolves completely in DMSO at a rapid rate. And gamma globulin is unaffected to up to 30% DMSO dilution in carbonate buffer. Tetramethylrhodamine isothiocyanate doesn’t dissolve in other denaturing agents completely.

Application

Tetramethylrhodamine isothiocyanate or TRITC is used for tracking the pathway of calcium phosphate/DNA nanoparticles during cell transfection. It is an extremely intensive fluorochrome used for labeling antibodies.

Other Notes

An extremely intensive fluorochrome for labeling antibodies

pictograms

Health hazardExclamation mark
GHS08,GHS07

signalword

Danger

Hazard Classifications

Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - Skin Sens. 1 - STOT SE 3

target_organs

Respiratory system

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

dust mask type N95 (US), Eyeshields, Faceshields, Gloves

Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

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Visite la Librería de documentos

Haaijman, J.J.
IBRO Hand. Ser., 3, 47-47 (1983)
Viktoriya Sokolova et al.
Journal of biological inorganic chemistry : JBIC : a publication of the Society of Biological Inorganic Chemistry, 12(2), 174-179 (2006-10-13)
Calcium phosphate nanoparticles were prepared by precipitation from water and were then functionalized by DNA. These particles are taken up by living cells and function as gene transfer agents, i.e., the DNA is brought into a cell's nucleus and is
The conjugation of immunoglobulins with tetramethylrhodamine isothiocyanate by utilization of dimethylsulfoxide (DMSO) as a solvent.
N R Bergquist et al.
Journal of immunological methods, 5(2), 189-198 (1974-07-01)
The conjugation of immunoglobulins with tetramethylrhodamine isothiocyanate. A comparison between the amorphous and the crystalline fluorochrome.
L Amante et al.
Journal of immunological methods, 1(3), 289-301 (1972-05-01)

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