79454

Ampliflu Red Westernblot Kit

for fluorescent detection

• UNSPSC Code: 12352200

Propiedades

• usage: sufficient for 50 applications
• fluorescence: λ_ex 571 nm; λ_em 585 nm
• storage temp.: 2-8°C

Descripción

General description

<p>find more information here</p>

Application

The Ampliflu Red Western Blot Kit provides an alternative to the classical chemiluminescence detection. When working with Ampliflu Red, standard protocols for the immunoblotting-procedure can be used until the detection step. No extra time for signal accumulation is necessary as it is for chemiluminescent signals. The use of Ampliflu Red is suitable for protein quantities between 1 ng and 1 μg per band of protein.

Biochem/physiol Actions

The kit contains the peroxidase substrate Ampliflu Red that is enzymatically converted to the highly fluorescent compound, resorufin. Fluorescent imaging is then performed on the immunoblot.

Analysis Note

Sensitivity: Limit of detection is 1 ng/band, but may vary depending on the antibodies used.

Legal Information

Ampliflu is a trademark of Sigma-Aldrich Co. LLC

Solo componentes del kit

• Dimethyl sulfoxide, puriss. p.a., ACS reagent, ≥99.9% (GC) 3 mL

Los componentes del kit también están disponibles por separado

• 90101

  Ampliflu^™ Red, suitable for fluorescence, ≥98.0% (HPLC) 5 vial(s)

  SDS
• 79382

  Phosphate buffered saline, tablet 5 tablets

  SDS

Información de seguridad

• pictograms: GHS07
• signalword: Warning
• hcodes: H302
• pcodes: P264 - P270 - P301 + P312 - P501
• Hazard Classifications: Acute Tox. 4 Oral
• Clase de almacenamiento: 10 - Combustible liquids
• flash_point_f: Not applicable
• flash_point_c: Not applicable