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47671

Sigma-Aldrich

Formamida

BioUltra, for molecular biology, ≥99.5% (T)

Sinónimos:

Amida fórmica, Amidas C1

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250 ML
$175.00
1 L
$551.00
2.5 L
$1,010.00

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Disponible para envío el07 de abril de 2025Detalles

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250 ML
$175.00
1 L
$551.00
2.5 L
$1,010.00

About This Item

Fórmula lineal:
HCONH2
Número de CAS:
75-12-7
Peso molecular:
45.04
Beilstein:
505995
Número CE:
200-842-0
Número MDL:
MFCD00007941
Código UNSPSC:
12352111
ID de la sustancia en PubChem:
57651075
NACRES:
NA.25
bp:
210 °C (lit.)

$175.00

Disponible para envío el07 de abril de 2025Detalles

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grado

Molecular Biology
for molecular biology

densidad de vapor

1.55 (vs air)

presión de vapor

0.08 mmHg ( 20 °C)
30 mmHg ( 129 °C)

Línea del producto

BioUltra

Ensayo

≥99.5% (T)

Formulario

liquid

temp. de autoignición

932 °F

técnicas

FISH: suitable
hybridization: suitable
protein extraction: suitable

impurezas

DNases, none detected
 RNases, none detected
 insoluble matter, passes filter test
 phosphatases, none detected
 proteases, none detected

residuo de ign.

≤0.1%

índice de refracción

n20/D 1.447 (lit.)
n20/D 1.447

pH

7-9 (25 °C, 1 M in H2O)

bp

210 °C (lit.)

mp

2-3 °C (lit.)

solubilidad

H2O: 10 M at 20 °C, clear, colorless

densidad

1.134 g/mL at 25 °C (lit.)

trazas de anión

chloride (Cl-): ≤1 mg/kg
sulfate (SO42-): ≤10 mg/kg

trazas de catión

Ag: ≤5 mg/kg
Al: ≤1 mg/kg
As: ≤0.1 mg/kg
Ba: ≤1 mg/kg
Bi: ≤1 mg/kg
Ca: ≤5 mg/kg
Cd: ≤1 mg/kg
Co: ≤1 mg/kg
Cr: ≤1 mg/kg
Cu: ≤1 mg/kg
Fe: ≤1 mg/kg
K: ≤20 mg/kg
Li: ≤1 mg/kg
Mg: ≤1 mg/kg
Mn: ≤1 mg/kg
Mo: ≤1 mg/kg
NH4+: ≤1000 mg/kg
Na: ≤20 mg/kg
Ni: ≤1 mg/kg
Pb: ≤1 mg/kg
Sr: ≤1 mg/kg
Tl: ≤5 mg/kg
Zn: ≤1 mg/kg

cadena SMILES

NC=O

λ

10 M in H2O

Absorción UV

λ: 260 nm Amax: ≤0.4
λ: 280 nm Amax: ≤0.1

aplicaciones

genomic analysis

InChI

1S/CH3NO/c2-1-3/h1H,(H2,2,3)

Clave InChI

ZHNUHDYFZUAESO-UHFFFAOYSA-N

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Descripción general

Formamide, also referred to as Carbamaldehyde, is an amide compound derived from formic acid. It plays a crucial role in biochemical research, particularly in denaturation and renaturation of nucleic acids (RNA and DNA). This includes applications like hybridization, sequencing gel electrophoresis, and electron microscopy. In molecular biology, BioUltra Formamide finds extensive use in various applications such as nucleic acid hybridization buffers, genetic fingerprinting, Southern and Northern blotting, as well as YAC screening. It typically functions by lowering the melting temperature of nucleic acid hybrids, facilitating their manipulation, and can enhance denaturation conditions in sequencing gels for more robust results.

Aplicación

La formamida desestabiliza los dúplex de ácidos nucleicos y puede utilizarse, normalmente, a una concentración del 50 % en protocolos de hibridación que requieran temperaturas de hibridación mas bajas.
Formamide has been used:
  • as a component in the hybridization buffer for in situ hybridization as a component of solution X to wash the slides to remove the coverslips
  • in the preparation of denaturation solution for spectral karyotyping (SKY) analysis
  • in the preparation of 1% formaldehyde solution for the pretreatment of slides for comparative genomic hybridization (CGH)
  • to prepare pre-hybridization buffer I, II, III, IV, and hybridization buffer I, II, and III for RNA fluorescence in situ hybridization (RNA-FISH)
  • as an ionizing solvent in the production of formic esters and vitamin, a softener for paper, in animal glues, and water-soluble gums, in molecular biology as a denaturing agent

Acciones bioquímicas o fisiológicas

Formamide serves as a good solvent for polar organic and inorganic compounds with potential catalytic activity in prebiotic processes due to its high dielectric constant.

Características y beneficios

  • Versatile and adaptable for a wide variety of laboratory and research applications
  • BioUltra Grade for your Molecular Biology, Cell Biology and Biochemical research
  • Tested to confirm low levels of heavy metal contamination, ensuring suitability for various applications
  • Free from DNase, RNase, NICKase and proteaseTested for trace levels of Anions and Cations

Otras notas

For additional information on our range of Biochemicals, please complete this form.
For the preparation of formamide denaturing polyacrylamide gels[1]; In situ hybridization on human chromosomes[2]

Pictogramas

Health hazard
GHS08

Palabra de señalización

Danger

Frases de peligro

H351,H360D,H373

Clasificaciones de peligro

Carc. 2 - Repr. 1B - STOT RE 2 Oral

Órganos de actuación

Blood

Código de clase de almacenamiento

6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

Clase de riesgo para el agua (WGK)

WGK 1

Punto de inflamabilidad (°F)

305.6 °F

Punto de inflamabilidad (°C)

152 °C

Equipo de protección personal

Eyeshields, Gloves, type ABEK (EN14387) respirator filter

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Certificados de análisis (COA)

Lot/Batch Number
BCCM8945
BCCM8946
BCCL7650
BCCL7647
BCCJ8866

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Si necesita una versión concreta, puede buscar un certificado específico por el número de lote.

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Encuentre la documentación para los productos que ha comprado recientemente en la Biblioteca de documentos.

Visite la Librería de documentos

R.C. Ogden et al.
Methods in Enzymology, 152, 67-67 (1987)
G. Wilkinson, ed.
In Situ Hybridization: A practical approach, 147-147 (1992)
Min Chen et al.
Prenatal diagnosis, 35(6), 534-543 (2015-02-03)
To develop a single-tube multi-marker assay for improved preimplantation genetic diagnosis (PGD) of deletional and/or non-deletional Hb Bart's hydrops fetalis syndrome, providing haplotype confirmation of deletional status, and maximization of linkage informativity. We performed in silico mining to identify novel
A Touati et al.
Journal of clinical microbiology, 53(10), 3182-3194 (2015-07-24)
Molecular typing of Mycoplasma pneumoniae is an important tool for identifying grouped cases and investigating outbreaks. In the present study, we developed a new genotyping method based on single nucleotide polymorphisms (SNPs) selected from the whole-genome sequencing of eight M.
Jing Wang et al.
The journal of physical chemistry. B, 117(8), 2314-2320 (2013-01-26)
A step-by-step mechanistic pathway following the transformation of formamide to purine through a five-membered ring intermediate has been explored by density functional theory computations. The highlight of the mechanistic route detailed here is that the proposed pathway represents the simplest
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Questions

1–9 of 9 Questions  

  1. What temperature should this product be stored at?

    1 answer

    1. This product may be stored at room temperature.

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  2. What temperature should this product be stored at?

    1 answer

    1. This product may be stored at room temperature.

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  3. How is shipping temperature determined? And how is it related to the product storage temperature?

    1 answer

    1. Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf

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  4. How can I determine the shelf life / expiration / retest date of this product?

    1 answer

    1. If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
      For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
      For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/449/386/product-dating-information-mk.pdf

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  5. What expiry does Formamide get?

    1 answer

    1. The product specification sheet for this product contains a recommended retest date period of roughly 11 months after the quality release date. A recommended retest date is the period of time during which the product is expected to remain within established stability specifications, provided that it has been stored under defined conditions. After the Retest Date, product samples should be examined to ensure that the product is still in compliance with the established specifications. For more information, you may access the "Product Dating Information" document under "ADDITIONAL USEFUL DOCUMENTS ABOUT OUR PRODUCTS" at the bottom of the Quality Services page with this link: https://www.sigmaaldrich.com/US/en/life-science/quality-and-regulatory-management/quality-services.

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  6. What is the Department of Transportation shipping information for this product?

    1 answer

    1. Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.

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  7. What is the best way to deionize Formamide, Product 47671?

    1 answer

    1. Mixed Bed Resin (e.g., Sigma Product No. M8157 or M8032) can be used to deionize formamide.

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  8. What is the molarity of the neat liquid Formamide, Product 47671?

    1 answer

    1. Based on the density of 1.13 and the molecular weight of 45.04, the molarity of this neat liquid is 25.09 M. The pH of a 0.5 M aqueous solution is 7.1, according to the chemicals encyclopedia published by the Royal Society of Chemistry, 12th edition.

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  9. Are aqueous solutions of Formamide, Product 47671, stable?

    1 answer

    1. In aqueous solution, formamide hydrolyzes to form ammonia and formic acid (i.e., ammonium formate).

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