01824
Acylase I, immobilized on Eupergit® C from Aspergillus sp.
≥50 U/g moist material
Sinónimos:
Aminoacylase, immobilized, Plexazym® AC
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About This Item
MDL number:
UNSPSC Code:
12352204
Productos recomendados
form
beads
specific activity
≥50 U/g moist material
storage temp.
2-8°C
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General description
the immobilized acylase catalyzes the hydrolysis of N-acetyl-DL-amino acid to L-amino acid, the D-form is not attacked
Unit Definition
1 U corresponds to the amount of enzyme which hydrolyzes 1 μmol N-acetyl-L-methionine per minute at pH 8.0 and 25°C
Reconstitution
Standard procedure: a 10-20% substrate solution, pH 6-8, with an addition of CoCl2 (10-4 moles) at 33°C was used. Prior to use the polymer was washed with water (50 times bed volumes); when used in a fixed bed reactor, a velocity of flow of 3 bed volumes/h leads to a hydrolysis degree of 80%
Analysis Note
moist pearls (dried substance ~30%, pearl diameter 50-100 μm), covalent fixation of the acylase
Other Notes
The immobilized acylase is used for the convenient resolution of amino acids via the selective deacetylation of N-acetyl-L-amino acids in DL-racemates
Legal Information
Eupergit is a registered trademark of Röhm GmbH & Co. KG
Plexazym is a registered trademark of Röhm GmbH & Co. KG
Storage Class
13 - Non Combustible Solids
wgk_germany
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
Certificados de análisis (COA)
Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»
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J. Tramper
Solid Phase Biochemistry, 393-393 (1983)
Preparation and properties of enzymes immobilized by copolymerization.
D Jaworek et al.
Methods in enzymology, 44, 195-120 (1976-01-01)
W. Kuhlmann et al.
Chemie Ingenieur Technik, 52, 607-607 (1980)
Optical resolution of racemic amino acids by aminoacylase.
T Sato et al.
Bioprocess technology, 16, 3-14 (1993-01-01)
Tao Dong et al.
Bioresource technology, 101(16), 6569-6571 (2010-04-07)
The effects of bovine serum albumin (BSA) addition on the cross-linked enzyme aggregates (CLEA) of aminoacylase from Aspergillus melleus (EC 3.5.1.14) were conducted at varying glutaraldehyde to enzyme ratio. After optimization, CLEA of aminoacylase prepared with 10 mg BSA per
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