PPB019

Bis-Tris Bicine EDTA buffer

pH 7.2, pHast Pack^™, powder

• Sinónimos: 1X Bis-Tris transfer buffer, Bis-Tris
• Código UNSPSC: 41105319
• NACRES: NA.25

Propiedades

• Línea del producto: pHast Pack^™
• Nivel de calidad: 100
• Formulario: powder
• condiciones de almacenamiento: (Tightly closed. Dry)
• pH: 7.2
• trazas de catión: Pb: ≤5 ppm
• actividad extraña: DNAse, free; NICKase, free; Protease, free; RNAse, free
• temp. de almacenamiento: room temp

Descripción

Descripción general

Bis-Tris Bicine EDTA buffer or Bis-Tris buffer (pH 7.2) contains Bis-Tris and Bicine, zwitterionic buffer components that form strong bonds with common metals. In combination with ethylenediaminetetraacetic acid (EDTA), a metal chelator, that binds divalent cations. This buffer is commonly used as a transfer buffer in western blot to transfer proteins from the gel to the membrane.

Aplicación

Bis-Tris buffer may be used in western blot as a transfer buffer.

Características y beneficios

• Ready-to-use buffer
• No measuring and pH adjustment needed
• Certified quality for reproducibility
• Minimizes exposure to toxic chemicals
• Biological tests: free of DNase, RNase, Protease, and Nickase
• Chemical tests: Iron ≤10 ppm, Lead ≤5 ppm

Envase

Foil pouches

Reconstitución

Contents of one pouch, when dissolved in 500 mL of ultrapure water, will yield a 1X solution containing 25 mM Bicine, 25 mM Bis-Tris, 1 mM EDTA, pH 7.2 at 25 °C. Contents tested to be DNAse, RNAse, Protease, and Nickase free.

Almacenamiento y estabilidad

Store at room temperature. Product may naturally agglomerate but can be simply broken up within the pouch prior to use.

Otras notas

For additional information on our range of Biochemicals, please complete this form.

Información legal

pHast Pack is a trademark of Sigma-Aldrich Co. LLC

Información de seguridad

• Pictogramas: GHS05
• Palabra de señalización: Danger
• Frases de peligro: H318
• Consejos de prudencia: P280 - P305 + P351 + P338
• Clasificaciones de peligro: Eye Dam. 1
• Código de clase de almacenamiento: 11 - Combustible Solids
• Clase de riesgo para el agua (WGK): WGK 3