11758241001
Roche
β-Gal Reporter Gene Assay, chemiluminescent
sufficient for 500 assays (microplate), sufficient for 250 assays (tubes)
Sinónimos:
galactosidase
Iniciar sesiónpara Ver la Fijación de precios por contrato y de la organización
About This Item
UNSPSC Code:
23201100
Productos recomendados
usage
sufficient for 250 assays (tubes)
sufficient for 500 assays (microplate)
manufacturer/tradename
Roche
storage temp.
2-8°C
General description
The β-Gal Reporter Gene Assay is designed for specifically measuring bacterial β-galactosidase activity. To achieve this, the enzyme reaction is conducted at a pH that is optimized for bacterial β-Gal, but allows no significant endogenous eukaryotic β-galactosidase activity. However, if very high endogenous β-galactosidase activity is found (e.g., with hepatic cells), heat treatment can be performed to ensure the specific determination of the amount of bacterial β-galactosidase that is encoded by the transfected plasmid.
Application
The β-Gal Reporter Gene Assay, chemiluminescent, is used to quantitatively measure β-Gal expression in eukaryotic cells that are transfected with a plasmid bearing the β-Gal-encoding lacZ reporter gene.
Features and Benefits
- Sensitive: Chemiluminescence technology allows detection of approximately 20 fg β-galactosidase in cell extracts
- High dynamic measuring range: Linear range over four to five orders of magnitude
- Constant light emission: The assay produces a long-lasting light emission instead of a short-peak kinetic
- Safe: No radioactive isotopes are used
- Fast: Approximately 1.5 to 2.5 hours elapse from start to finish
- Convenient: Kit contains all reagents needed, including lysis buffer, protease inhibitors, and a positive control
Packaging
1 kit containing 7 components.
Preparation Note
Storage conditions (working solution): Substrate Reagent (solution 1) is stable at least for 24 h at 2 to 8 °C.
Initation Reagent (solution 2) is stable for at least 4 weeks when stored at 2 to 8 °C.
Lysis Reagent, 1x (solution 3) Lysis reagent without protease inhibitors is stable at
-15 to -25 °C or for 3 months at 2 to 8 °C. Lysis reagent containing protease inhibitors is stable for one week when stored frozen at -15 to -25 °C.
Positive Control (solution 4) The reconstituted enzyme solution is stable for 1 week at 2 to 8 °C. When stored frozen in aliquots at -15 to -25 °C the enzyme is stable for 3 months. Avoid multiple freezing and thawing.
Initation Reagent (solution 2) is stable for at least 4 weeks when stored at 2 to 8 °C.
Lysis Reagent, 1x (solution 3) Lysis reagent without protease inhibitors is stable at
-15 to -25 °C or for 3 months at 2 to 8 °C. Lysis reagent containing protease inhibitors is stable for one week when stored frozen at -15 to -25 °C.
Positive Control (solution 4) The reconstituted enzyme solution is stable for 1 week at 2 to 8 °C. When stored frozen in aliquots at -15 to -25 °C the enzyme is stable for 3 months. Avoid multiple freezing and thawing.
Other Notes
For life science research only. Not for use in diagnostic procedures.
Solo componentes del kit
Referencia del producto
Descripción
- β-Gal Substrate, Galacton Plus 100x concentrated
- Assay Buffer
- Enhancer
- Initiation Solution
- Protease Inhibitors
- Lysis Buffer 5x concentrated
- Positive Control, β-galactosidase (E. coli), lyophilizate
related product
Referencia del producto
Descripción
Precios
signalword
Danger
Hazard Classifications
Aquatic Chronic 3 - Eye Dam. 1 - Flam. Liq. 2 - Met. Corr. 1 - Skin Corr. 1B - Skin Sens. 1 - STOT SE 3
target_organs
Central nervous system
Storage Class
3 - Flammable liquids
wgk_germany
WGK 2
Certificados de análisis (COA)
Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»
¿Ya tiene este producto?
Encuentre la documentación para los productos que ha comprado recientemente en la Biblioteca de documentos.
Molecular Analysis of the HOXA2-Dependent Degradation of RCHY1
Bridoux L, et al.
PLoS ONE (2015)
Alexandra Tauzin et al.
Journal of virology, 95(15), e0231120-e0231120 (2021-05-14)
Type I interferons (IFNs) are a family of cytokines that represent a first line of defense against virus infections. The 12 different IFN-α subtypes share a receptor on target cells and trigger similar signaling cascades. Several studies have collectively shown
HOXA2 activity regulation by cytoplasmic relocation, protein stabilization
and post-translational modification
and post-translational modification
Deneyer, et al.
Biochimica et Biophysica Acta (BBA)-Proteins and Proteomics (2019)
The O-GlcNAc transferase OGT interacts with and post-translationally modifies the transcription factor HOXA1
Draime et al.
Febs Letters (2018)
Arnaud Taminiau et al.
Nucleic acids research, 44(15), 7331-7349 (2016-07-07)
HOX proteins define a family of key transcription factors regulating animal embryogenesis. HOX genes have also been linked to oncogenesis and HOXA1 has been described to be active in several cancers, including breast cancer. Through a proteome-wide interaction screening, we
Nuestro equipo de científicos tiene experiencia en todas las áreas de investigación: Ciencias de la vida, Ciencia de los materiales, Síntesis química, Cromatografía, Analítica y muchas otras.
Póngase en contacto con el Servicio técnico