11427857910

Fluorescein-12-UTP

≥85% (HPLC), solution

• Sinónimos: Fluorescein-12-UTP tetralithium salt, Fluorescein-5(6)-carboxamidocaproyl-(5-[3-aminoallyl]uridine 5′-triphosphate)
• Fórmula empírica (notación de Hill): C₃₉H₄₁N₄O₂₂P₃
• Número de CAS: 134367-01-4
• Peso molecular: 1010.68
• Número MDL: MFCD00866062
• Código UNSPSC: 41116100

Propiedades

• Nivel de calidad: 100
• Ensayo: ≥85% (HPLC)
• Formulario: solution
• mol peso: 1034.4
• envase: pkg of 25 μL (250 nmol; 10 mM)
• fabricante / nombre comercial: Roche
• temp. de almacenamiento: −20°C

Descripción

Descripción general

Quality
Typical analysis: At least 85% Fluorescein-12-UTP (HPLC, area%).

Fluorescein is bound to uridine triphosphate via an amide linkage. Molecular formula is C₃₉H₃₇N₄O₂₂P₃Li₄.

Aplicación

Fluorescein-12-UTP is used as a substrate for SP6, T3, and T7 RNA polymerases. It can replace UTP in the in vitro transcription reaction for RNA labeling.[1]
Labeled RNA can be subsequently detected by in situ hybridization and direct fluorescence detection or detection by ELISA using Anti-Fluorescein-AP, Fab fragments or Anti-Fluorescein-POD, Fab fragments.

Otras notas

For life science research only. Not for use in diagnostic procedures.

For your convenience, fluorescein-UTP is also available in a convenient labeling mix (single solution with conventional NTPs and Fluorescein-12-UTP).

Información de seguridad

• Código de clase de almacenamiento: 12 - Non Combustible Liquids
• Clase de riesgo para el agua (WGK): nwg
• Punto de inflamabilidad (°F): does not flash
• Punto de inflamabilidad (°C): does not flash