11214667001
Roche
Anti-Digoxigenin, Fab fragments
from sheep
Sinónimos:
anti-digoxigenin, digoxigenin
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About This Item
Código UNSPSC:
12352203
Productos recomendados
origen biológico
sheep
Nivel de calidad
conjugado
unconjugated
forma del anticuerpo
affinity purified immunoglobulin
tipo de anticuerpo
primary antibodies
clon
polyclonal
Formulario
lyophilized
envase
pkg of 1 mg
fabricante / nombre comercial
Roche
técnicas
ELISA: 2-4 μg/mL
immunohistochemistry: 0.5-1 μg/mL
western blot: 0.5-2 μg/mL
isotipo
IgG1
temp. de almacenamiento
2-8°C
Descripción general
Anti-Digoxigenin, Fab fragments are Fab fragments from polyclonal anti-digoxigenin antibodies. Anti-Digoxigenin Fab fragments are preferentially used for secondary detection using a light chain specific anti-sheep conjugate.
Digoxigenin is a hapten, useful in labeling nucleic acids and in detection systems.[1]
Especificidad
The polyclonal antibody from sheep is specific to digoxigenin and digoxin and shows no cross-reactivity with other steroids, such as human estrogens and androgens.
Aplicación
Anti-Digoxigenin Fab fragments are preferentially used for secondary detection using a light chain specific anti-sheep conjugate.
Use Anti-Digoxigenin, Fab fragments for the detection of digoxigenin-labeled compounds using:
Use Anti-Digoxigenin, Fab fragments for the detection of digoxigenin-labeled compounds using:
Características y beneficios
Specific to digoxigenin and digoxin.
Secuencia
The number of amino groups in the Fab fragments is not known, because polyclonal antibodies are not sequenced. But as a rule of thumb full length antibodies contain about 80 amino groups on average.
Nota de preparación
Working concentration: Working concentration of antibody depends on application and substrate. The following concentrations should be taken as a guideline:
Storage conditions (working solution): Diluted antibody is stable at 2 to 8 °C for 12 hours.
Always prepare freshly!
- ELISA: 2 to 4 μg/ml
- Immunohistocytochemistry: 0.5 to 1 μg/ml
- In situ hybridization: 0.2 to 0.4 μg/ml
- Western blot: 0.5 to 2 μg/ml
Storage conditions (working solution): Diluted antibody is stable at 2 to 8 °C for 12 hours.
Always prepare freshly!
Reconstitución
Add 1 ml PBS to a final concentration of 1 mg/ml. Centrifuge reconstituted antibody for 5 minutes at 10,000 rpm prior to each use, and pipet the necessary amount carefully from the surface.
Nota de análisis
Preparation: After immunization with digoxigenin, sheep IgGs are purified by ion exchange chromatography. Specific IgGs are isolated by immunosorption. Fab fragments obtained after papain digestion are purified by gel filtration and lyophilized.
Otras notas
For life science research only. Not for use in diagnostic procedures.
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Código de clase de almacenamiento
11 - Combustible Solids
Clase de riesgo para el agua (WGK)
WGK 1
Punto de inflamabilidad (°F)
does not flash
Punto de inflamabilidad (°C)
does not flash
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