SCC262

SK-MEL-37 Human Melanoma Cell Line

• NACRES: NA.81
• UNSPSC Code: 41106514
• Biological source: human
• Growth mode: N/A
• Technique(s): cell culture | mammalian: suitable

Propiedades

• biological source: human
• Quality Segment: 100
• packaging: vial of >1X10⁶ cells
• manufacturer/tradename: Millipore
• growth mode: N/A
• technique(s): cell culture | mammalian: suitable
• shipped in: liquid nitrogen
• storage temp.: −196°C

Descripción

Application

• Each vial contains > 1X106 viable cells.
• Cells are tested negative for infectious diseases by a Human Essential CLEAR panel by Charles River Animal Diagnostic Services.
• Cells are verified to be of human origin and negative for inter-species contamination from mouse, rat, chinese hamster, Golden Syrian hamster, and Non-human Primate (NHP) as assessed by a Contamination Clear panel by Charles River Animal Diagnostic Services
• Cells are negative for mycoplasma contamination.

Malignant melanoma is the most aggressive type of skin cancer, and the fifth most common cancer in the Unites States.1 As survival rate depends on the stage of disease at time of diagnosis, early detection is crucial to improving disease outcome and saving lives. Amelanotic (or hypomelanotic) melanoma (AM) is a rare subtype of cutaneous melanoma, comprising between 2-8% of total cases.2 AM is characterized by decreased or null presence of melanin, due to loss of pigment in tumor evolution. AM is usually diagnosed at more advanced stages, resulting in lower overall survival. AM is frequently observed in patients with melanocortin 1 receptor gene (MC1R) genotypes linked to certain phenotypes such as red hair.2
The SK-MEL-37 amelanotic cutaneous melanoma cell line is a well-established model for human melanoma, widely utilized in investigations into the mutational landscape of melanoma and the enhanced antigen expression that occurs concomitantly with melanoma progression. SK-MEL-37 expresses the melanoma-associated antigens MAGE-A1, tyrosinase, and p97, and cell surface antigens essential for metastasis such as CD71 and CD146.3 SK-MEL-37 is valuable and highly characterized cell system for transformation of melanocytes into melanoma and identification of therapeutic targets for this disease.

Source
SK-MEL-37 was established from a metastatic lymph node of a 65-year old male patient with malignant melanoma of rectal tumor origin.4

References
1. CA Cancer J Clin 2019, 69(1): 7-34.
2. Cancers (Basel) 2020, 12(9): 2362.
3. J Cancer 2013, 45(5): 371-382.
4. J Exp Med 1976, 144(4): 873

Features and Benefits

SK-MEL-37 is valuable and highly characterized cell system for transformation of melanocytes into melanoma and identification of therapeutic targets for this disease.

Preparation Note

The cells should be stored in liquid nitrogen until use. The cells can be cultured for at least 10 passages after initial thawing without significantly affecting functionality.

Other Notes

Subject to local law, this product is intended to be sold for internal in vitro research use only subject to terms and conditions found here: www.sigmaaldrich.com/restrictedcelluse. This product may not be: re-engineered or copied; used to make derivatives, modifications or functional equivalents; used to obtain patents or other IP claiming use of the product; used to develop, test, or manufacturer a commercial product; used as a component in a commercial product; resold or licensed; used in any clinical applications or trials; or used in humans. A license or limited commercial use agreement is required for use by any for-profit entity, use in services, and use in sponsored academic research. For information regarding any such use, please contact licensing@milliporesigma.com.

Disclaimer

RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Información de seguridad

• Clase de almacenamiento: 10 - Combustible liquids
• ¿Qué está pasando?: WGK 2
• Punto de inflamación (°F): Not applicable
• Punto de inflamación (°C): Not applicable