SCC129
HT-22 Mouse Hippocampal Neuronal Cell Line
Mouse
Sinónimos:
HT22 Cell Line
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About This Item
UNSPSC Code:
41106514
eCl@ss:
32011203
NACRES:
NA.81
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product name
HT-22 Mouse Hippocampal Neuronal Cell Line, HT-22 mouse neuronal cell line is a valuable cell model for studies of glutamate-induced toxicity in neuronal cells.
biological source
mouse
Quality Level
technique(s)
cell based assay: suitable
shipped in
dry ice
General description
Glutamate is a major excitatory neurotransmitter used as a signaling molecule between nerve cells and is involved in most aspects of normal brain function including cognition, learning and memory. Various glutamate receptors are found throughout the brain and include NMDA, AMPA/kainate and metabotropic receptors (mGluR). Normal glutamate levels are essential for normal brain function, however high levels may result in over-excitation of nerve cells, leading to eventual cell damage and/or cell death. Glutamate-induced cytotoxicity has been implicated in neurodegenerative disorders such as Alzheimer’s disease, Huntington’s disease and Parkinson’s disease along with other conditions including spinal cord injury and multiple sclerosis. Therapies that may inhibit or reduce the effects of glutamate activity are thus of great therapeutic interest.
HT-22 is an immortalized mouse hippocampal cell line subcloned from the HT-4 cell line . The parental HT-4 cell line was derived from the immortalization of mouse neuronal tissues with a temperature sensitive SV40 T-antigen . HT-22 is highly sensitive to glutamate and is thus frequently used as a model system to study glutamate-induced toxicity in neuronal cells.
Cell Line Description
Neural Lineage Cells
Application
EMD Millipore has a limited license to sell this product for research use only, and not for any commercial uses. Excluded commercial uses include without limitation manufacturing, providing a service, therapeutic, diagnostic and prophylactic uses, and any other commercial uses. Use of this product by a purchaser for any purpose other than for research is unauthorized and prohibited.
Inquiries about licensing for commercial or other uses should be directed to:
Office of Technology Development
The Salk Institute for Biological Studies
10010 North Torrey Pines Road
La Jolla, CA 92037
Phone: (858) 453-4100 extension 1278
Email: OTD@salk.edu
Inquiries about licensing for commercial or other uses should be directed to:
Office of Technology Development
The Salk Institute for Biological Studies
10010 North Torrey Pines Road
La Jolla, CA 92037
Phone: (858) 453-4100 extension 1278
Email: OTD@salk.edu
Research Category
Neuroscience
Toxicity
Neuroscience
Toxicity
Quality
• Each vial contains ≥ 1X106 viable cells.
• Cells are tested negative for infectious diseases by a Mouse Essential CLEAR panel by Charles River Animal Diagnostic Services.
• Cells are verified to be of mouse origin and negative for inter-species contamination from rat, chinese hamster, Golden Syrian hamster, human and non-human primate (NHP) as assessed by a Contamination Clear panel by Charles River Animal Diagnostic Services.
• Cells are negative for mycoplasma contamination.
• Cells are tested negative for infectious diseases by a Mouse Essential CLEAR panel by Charles River Animal Diagnostic Services.
• Cells are verified to be of mouse origin and negative for inter-species contamination from rat, chinese hamster, Golden Syrian hamster, human and non-human primate (NHP) as assessed by a Contamination Clear panel by Charles River Animal Diagnostic Services.
• Cells are negative for mycoplasma contamination.
Storage and Stability
Store in liquid nitrogen. The cells can be cultured for at least 10 passages after initial thawing without significantly affecting the cell marker expression and functionality.
Storage Class
12 - Non Combustible Liquids
wgk_germany
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
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Experimental & molecular medicine, 53(7), 1134-1147 (2021-07-09)
Calbindin, a major Ca2+ buffer in dentate granule cells (GCs), plays a critical role in shaping Ca2+ signals, yet how it regulates neuronal function remains largely unknown. Here, we found that calbindin knockout (CBKO) mice exhibited dentate GC hyperexcitability and
J B Davis et al.
Brain research, 652(1), 169-173 (1994-07-25)
A neuronal cell line, HT-22, is sensitive to glutamate cytotoxicity via a non-receptor mediated oxidative pathway. 12-O-tetradecanoylphorbol-13-acetate (TPA), an activator of protein kinase C, blocks this glutamate-induced cell death. Down-regulation of protein kinase C eliminates the protection against glutamate cytotoxicity
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Phosphodiesterase 4D (PDE4D) inhibitor is commonly used to treat depression, but side effects seriously decrease its efficacy. PDE4D was a downstream target mRNA of miR-139-5p. Therefore, we examined the effects of hippocampal miR-139-5p gain- and loss-of-function on depression-like behaviors, the
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Abortive cell cycle (ACC) re-entry of apoptotic neurons is a recently characterized phenomenon that occurs after central nervous system (CNS) injury or over the course of CNS disease. Consequently, inhibiting cell cycle progression is neuroprotective in numerous CNS pathology models.
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