OP13L
Anti-N-Myc Mouse mAb (NCM II 100)
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About This Item
forma del anticuerpo
purified antibody
Nivel de calidad
clon
NCM II 100, monoclonal
Formulario
lyophilized
reactividad de especies
human, mouse
isotipo
IgG1
Descripción general
Purified mouse monoclonal antibody derived by immunizing BALB/c mice with the specified immunogen and fusing splenocytes with SP2/0 mouse myeloma cells. Recognizes N-myc as well as its cleavage products.
Recognizes N-myc as well as its cleavage products in IMR5 cells. Myc is a labile protein. Fresh lysates in the presence of protease inhibitor cocktails are recommended.
The v-myc oncogene, initially identified in the MC29 avian retrovirus, causes myelocytomas, carcinomas, sarcomas and lymphomas, and belongs to a family of oncogenes conserved throughout evolution. In humans the family consists of five genes: c-myc, N-myc, R-myc, L-myc and B-myc. Amplification of the N-myc gene has been found in human neuroblastomas and cell lines. The extent of N-myc amplification correlates well with the stage of neuroblastoma disease. Immunological studies have shown that the human N-myc gene gives rise to at least two nuclear phosphoproteins of ~64 kDa and ~67 kDa that exhibit relatively short (30 min) half lives in vivo and exhibit DNA binding properties in vitro.
Inmunógeno
recombinant, human, N-Myc fusion protein, expressed in E. coli
Aplicación
Frozen Sections (1-5 μg/ml)
Immunoblotting (1-5 μg/ml, see application references)
Immunofluorescence (1-5 μg/ml, see application references)
Immunoprecipitation (1-2 μg/sample, see application references)
Immunoblotting (1-5 μg/ml, see application references)
Immunofluorescence (1-5 μg/ml, see application references)
Immunoprecipitation (1-2 μg/sample, see application references)
Forma física
Lyophilized from a volatile buffer, 100 μg BSA.
Reconstitución
Reconstitute the lyophilized antibody with 1 ml sterile PBS. Lyophilized antibodies should be reconstituted at 4°C with occasional gentle mixing for at least 2 h. Following reconstitution, refrigerate (4°C) for short-term storage or aliquot and freeze (-2
Nota de análisis
Positive Control
IMR5 cells
IMR5 cells
Otras notas
It is important to compensate for the short half life of this protein when preparing tissue sections or extracts. We recommended that all preparations be kept cold and that a cocktail of protease inhibitors be used. Antibody should be titrated for optimal
Código de clase de almacenamiento
10-13 - German Storage Class 10 to 13
Certificados de análisis (COA)
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Encuentre la documentación para los productos que ha comprado recientemente en la Biblioteca de documentos.
The myc oncogene: its role in transformation and differentiation.
M D Cole
Annual review of genetics, 20, 361-384 (1986-01-01)
N E Kohl et al.
Cell, 35(2 Pt 1), 359-367 (1983-12-01)
We have cloned a 2.0-kb EcoRI fragment of human genomic DNA (NB-19-21) which has homology to the v-myc oncogene but is distinct from the classical c-myc gene. This sequence is amplified from 25- to 700-fold in eight of nine tested
D J Slamon et al.
Science (New York, N.Y.), 232(4751), 768-772 (1986-05-09)
The human N-myc gene is related to the c-myc proto-oncogene, and has been shown to have transforming potential in vitro. Many studies have reported amplification of N-myc in human neuroblastoma and retinoblastoma cell lines. In primary tumors, amplification of the
R C Seeger et al.
The New England journal of medicine, 313(18), 1111-1116 (1985-10-31)
Eighty-nine patients with untreated primary neuroblastomas were studied to determine the relation between the number of copies of the N-myc oncogene and survival without disease progression. Genomic amplification (3 to 300 copies) of N-myc was detected in 2 of 16
R W Michitsch et al.
Molecular and cellular biology, 4(11), 2370-2380 (1984-11-01)
Screening of a partial cDNA library prepared from the human neuroblastoma cell line BE(2)-C with genomic DNA probes containing sequences representative of the amplified domain of that cell line allowed us to identify cloned transcripts from an active gene within
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