MABT528

Anti-Connexin 43 Antibody

mouse monoclonal, 1C5.1

• Sinónimos: Gap junction alpha-1 protein, Connexin-43, Cx43, Gap junction 43 kDa heart protein
• Código UNSPSC: 12352203
• eCl@ss: 32160702

Propiedades

• Nombre del producto: Anti-Connexin 43 Antibody, CT Antibody, clone 1C5.1, clone 1C5.1, from mouse
• origen biológico: mouse
• Nivel de calidad: 100
• forma del anticuerpo: purified immunoglobulin
• tipo de anticuerpo: primary antibodies
• clon: 1C5.1, monoclonal
• reactividad de especies: rat, human
• técnicas: immunohistochemistry: suitable (paraffin); western blot: suitable
• isotipo: IgG1κ
• Nº de acceso NCBI: NP_000156
• Nº de acceso UniProt: P17302
• Condiciones de envío: ambient
• modificación del objetivo postraduccional: unmodified
• Información sobre el gen: human ... GJA1(2697)

Descripción

Descripción general

Gap junction alpha-1 protein (UniProt P17302; also known as Connexin-43, Cx43, Gap junction 43 kDa heart protein) is encoded by the GJA1 (also known as AVSD3, CMDR, GJAL, HSS, ODDD) gene (Gene ID 2697) in human. A gap junction consists of a cluster of closely packed pairs of transmembrane channels, the connexins, through which materials of low molecular weight diffuse from one cell to a neighboring cell. Connexin-43 is a polytopic integral membrane protein oligomerizes into a hexamer or connexon. It is he most ubiquitously expressed connexin and contains a long C-terminal tail, an N-terminal domain, and multiple transmembrane domains. The C-terminal tail domain of human connexin-43 contains 50 amino acids and includes post-translational modification sites, as well as binding sites for transcription factors, cytoskeleton elements, and other proteins. The C-terminal tail regulates pH gating and channel assembly, whereas the N-terminal domain is involved in channel gating and oligomerization and controls the switch between channel open and closed states. Mutations in connexin-43 result in multiple disorders, such as oculodentodigital dysplasia that is characterized by microcornea, microphthalmia, and syndactyly of the fourth and fifth fingers. Connexin-43 is shown to negatively regulate growth of colon cancer cells, in part by enhancing apoptosis. Loss of connexin-43 expression in colorectal cancers is correlated with significantly shorter relapse-free and overall survival.

Ref.:
Sirnes, S et al. (2012). Int. J. Cancer 131, 570-581.
Naus, CC et al. (2010). Nat. Rev. Cancer. 10, 435-441.

Especificidad

Clone 1C5.1 targets an epitope within the second half of the C-terminal cytoplasmic tail.

Inmunógeno

GST-tagged recombinant human connexin 43 C-terminal cytoplasmic tail fragment.

Aplicación

Anti-Connexin 43, CT, clone 1C5.1, Cat. No. MABT528 is a mouse monoclonal antibody validated for use in Immunohistochemistry (Paraffin) and Western Blotting for the detection of connexin 43.

Immunohistochemistry Analysis: A 1:250-1,000 dilution from a representative lot detected cconnexin 43 in human cerebellum, heart muscle, and kidney tissue sections.

Calidad

Evaluated by Western Blotting in HUVEC lysate.

Western Blotting Analysis: 10 ng/mL of this antibody detected connexin 43 in 10 µg of HUVEC lysate.

Descripción de destino

~43 kDa observed. 42.88/42.90 kDa (human/rat) calculated (Met1 removed). Uncharacterized bands may be observed in some lysate(s).

Ligadura / enlace

Replaces: MAB3068

Forma física

Format: Purified

Otras notas

Concentration: Please refer to lot specific datasheet.

Información de seguridad

• Código de clase de almacenamiento: 12 - Non Combustible Liquids
• Clase de riesgo para el agua (WGK): WGK 1
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable