MABT1565

Anti-Desmin Antibody, clone DE-U-10

• Sinónimos: CMD1I, CSM1, CSM2
• Código UNSPSC: 12352203
• NACRES: NA.43

Propiedades

• origen biológico: mouse
• Nivel de calidad: 200
• forma del anticuerpo: purified antibody
• tipo de anticuerpo: primary antibodies
• clon: DE-U-10, monoclonal
• mol peso: calculated mol wt 53.6 kDa; observed mol wt ~16 kDa
• purificado por: using protein G
• reactividad de especies: hamster, human, rat, mouse
• reactividad de especies (predicha por homología): porcine
• envase: antibody small pack of 100
• técnicas: ELISA: suitable; immunofluorescence: suitable; western blot: suitable
• isotipo: IgG1κ
• secuencia del epítopo: C-terminal half
• Nº de acceso Protein ID: NP_001001535
• Nº de acceso UniProt: P02540
• temp. de almacenamiento: 2-8°C
• Información sobre el gen: Porcine ... DES(396725)

Descripción

Especificidad

Clone DE-U-10 is a mouse monoclonal antibody that detects Desmin. It targets an epitope within 48 amino acids from the C-terminal half of the protein.

Inmunógeno

Purified full length porcine Desmin.

Aplicación

Quality Control Testing

Evaluated by Western Blotting in mouse skeletal muscle tissue lysate.

Western Blotting Analysis: A 1:500 dilution of this antibody detected Desmin in mouse skeletal muscle tissue lysate.

Tested Applications

Western Blotting Analysis: A 1:500 dilution from a representative lot detected Desmin in human skeletal muscle tissue extracts.

ELISA Analysis: A representative lot detected Desmin in ELISA applications (Debus, E., et al. (1983). EMBO J. 2(12):2305-12).

Western Blotting Analysis: A representative lot detected Desmin in Western Blotting applications (Debus, E., et al. (1983). EMBO J. 2(12):2305-12).

Immunofluorescence Analysis: A representative lot detected Desmin in Immunofluorescence applications (Debus, E., et al. (1983). EMBO J. 2(12):2305-12).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

Descripción de destino

Desmin (UniProt: P02540) is encoded by the DES gene (Gene ID: 396725) in porcine. Desmin is a type III intermediate filament protein that is essential for maintaining the structural and functional integrity of muscle cells as part of the cytoskeletal network. It provides mechanical support to the muscle cells and facilitates the organization of contractile proteins within the cell. Additionally, it is involved in various cellular processes, including cell signaling, maintenance of cell shape, and intracellular transport. It is particularly abundant in cardiac, skeletal, and smooth muscle tissues. It is also enriched at postsynaptic membranes of neuromuscular junctions (NMJs). Mutations in the gene encoding Desmin have been associated with hereditary and sporadic myopathies, cardiomyopathies and myasthenic syndrome. (Ref.: Eiber, N., et al., (2020). Front. Mol. Neurosci. 13; Article 567084).

Forma física

Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Reconstitución

0.5 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Almacenamiento y estabilidad

Recommended storage: +2°C to +8°C.

Otras notas

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Información de seguridad

• Código de clase de almacenamiento: 12 - Non Combustible Liquids
• Clase de riesgo para el agua (WGK): WGK 1
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable