MABF2115
Anti-NKR-P1B (CD161b) Antibody, clone 2D12
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About This Item
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antibody form
purified antibody
clone
2D12
monoclonal
purified by
using protein G
species reactivity
mouse, mouse, mouse
concentration
(Please refer to lot specific datasheet.)
technique(s)
flow cytometry: suitable
immunoprecipitation (IP): suitable
western blot: suitable
isotype
IgG2aλ
suitability
suitable for immunoassay
UniProt accession no.
General description
Mouse BaF/3 cells (murine IL-3-dependent pro-B cell line) expressing mouse NKR-P1B protein.
Specificity
Clone 2D12 is a mouse monoclonal antibody that detects Natural killer cell surface protein NKR-P1B allele B6 (NKR-P1B) in murine species. It does not react with Nkrp1a, Nkrp1c, and Nkrp1f.
Application
Affects Function Analysis: A representative lot reversed the reduction in killing induced by the expression of Clrb in C1498, BW5147, and B6 LAK cells. (Iizuka, K., et. al. (2003). Nat Immunol. 4(8):801-7).
Immunoprecipitation Analysis: A representative lot immunoprecipitated NKR-P1B (CD161b) in Immunoprecipitation applications (Iizuka, K., et. al. (2003). Nat Immunol. 4(8):801-7).
Flow Cytometry Analysis: A representative lot detected NKR-P1B (CD161b) in Flow Cytometry applications (Iizuka, K., et. al. (2003). Nat Immunol. 4(8):801-7; Aguilar, O.A., et. al. (2017). Cell. 169(1):58-71).
Immunoprecipitation Analysis: A representative lot immunoprecipitated NKR-P1B (CD161b) in Immunoprecipitation applications (Iizuka, K., et. al. (2003). Nat Immunol. 4(8):801-7).
Flow Cytometry Analysis: A representative lot detected NKR-P1B (CD161b) in Flow Cytometry applications (Iizuka, K., et. al. (2003). Nat Immunol. 4(8):801-7; Aguilar, O.A., et. al. (2017). Cell. 169(1):58-71).
Quality
Evaluated by Western Blotting in mouse spleen tissue lysate.
Western Blotting Analysis: A 1:1,000 dilution of this antibody detected NKR-P1B (CD161b) in mouse spleen tissue lysate.
Western Blotting Analysis: A 1:1,000 dilution of this antibody detected NKR-P1B (CD161b) in mouse spleen tissue lysate.
Physical properties
~52 kDa observed; 25.03 kDa calculated. . Uncharacterized bands may be observed in some lysate(s).
Physical form
Purified mouse monoclonal antibody IgG2a in PBS without azide.
Storage and Stability
Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Certificados de análisis (COA)
Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»
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