MABE949

Anti-E3 Ubiquitin Ligase BRE1B Antibody, clone 4H8.1

clone 4H8.1, from mouse

• Sinónimos: E3 ubiquitin-protein ligase BRE1B, 95 kDa retinoblastoma-associated protein, BRE1-B, RBP95, RING finger protein 40, Syntaxin-1-interacting RING finger protein, Protein staring
• Código UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Propiedades

• origen biológico: mouse
• Nivel de calidad: 100
• forma del anticuerpo: purified immunoglobulin
• tipo de anticuerpo: primary antibodies
• clon: 4H8.1, monoclonal
• reactividad de especies: human
• técnicas: western blot: suitable
• isotipo: IgG1κ
• Nº de acceso NCBI: NP_001193962
• Nº de acceso UniProt: O75150
• modificación del objetivo postraduccional: unmodified
• Información sobre el gen: human ... RNF40(9810)

Descripción

Descripción general

E3 ubiquitin-protein ligase BRE1B (UniProt O75150; also known as 95 kDa retinoblastoma-associated protein, BRE1-B, RBP95, RING finger protein 40, Syntaxin-1-interacting RING finger protein, Protein staring) is encoded by the RNF40 (also known as BRE1B, KIAA0661) gene (Gene ID 9810) in human. Proteini ubiquitination is mediated by ubiquitin-activating enzymes (E1s), ubiquitin-conjugating enzymes (E2s), and ubiquitin ligases (E3s). There exist RING finger-type (really interesting new gene), HECT-type (homologous to E6-AP C-terminus), and U-box type of E3 ligases. RING finger ubiquitin ligase members can function as monomers, dimers, or multi-subunit complexes. The RNF20-RNF40 heterodimer mediates the ubiquitination of histone H2B at Lysine 120, an event that plays an important role in decompacting chromatin fiber to allow transcription elongation, in stem cell differentiation, as well as in DNA double-strand breaks (DSBs) repair via the homologous recombination (HR) and nonhomologous end-joining (NHEJ) pathways. Human RNF40 is a 1001-amino acid protein containing a RING-type zinc finger in its C-terminal region (a.a. 948-987) that mediates its interaction with RNF20.

Especificidad

Clone 4H8.1 epitope region is present in all three spliced isoforms of human BRE1B reported by UniProt (O75150).

Inmunógeno

Epitope: Internal (C-terminal half).

GST-tagged recombinant internal fragment from the C-terminal half of human BRE1B .

Aplicación

Anti-E3 Ubiquitin Ligase BRE1B Antibody, clone 4H8.1 is an antibody against E3 Ubiquitin Ligase BRE1B for use in Western Blotting.

Research Category
Epigenetics & Nuclear Function

Research Sub Category
Chromatin Biology

Calidad

Evaluated by Western Blotting in HeLa cell lysate.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected BRE1B in 10 µg of HeLa cell lysate.

Descripción de destino

~120 kDa oberved. 113.7 kDa (isoform 1), 94.80 kDa (isoform 2), 102.0 kDa (isoform 3) calculated.

Forma física

Format: Purified

Protein G Purified

Purified mouse monoclonal IgG1κ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Almacenamiento y estabilidad

Stable for 1 year at 2-8°C from date of receipt.

Otras notas

Concentration: Please refer to lot specific datasheet.

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Información de seguridad

• Código de clase de almacenamiento: 12 - Non Combustible Liquids
• Clase de riesgo para el agua (WGK): WGK 1
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable