MABE1020

Anti-Acetyl Lysine, Pan, clone RM101 Antibody

clone RM101, from rabbit, purified by affinity chromatography

• Código UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Propiedades

• origen biológico: rabbit
• Nivel de calidad: 100
• forma del anticuerpo: purified immunoglobulin
• tipo de anticuerpo: primary antibodies
• clon: RM101, monoclonal
• purificado por: affinity chromatography
• reactividad de especies: human
• reactividad de especies (predicha por homología): all (based on 100% sequence homology)
• técnicas: dot blot: suitable; immunocytochemistry: suitable; immunoprecipitation (IP): suitable; western blot: suitable
• isotipo: IgG
• Condiciones de envío: wet ice
• modificación del objetivo postraduccional: unmodified

Descripción

Descripción general

DNA is tightly packed in the nucleus into nucleosomes, generating an environment which is highly repressive towards DNA processes such as transcription. Acetylation of lysine residues within proteins has emerged as an important mechanism used by cells to overcome this repression. The acetylation of non-histone proteins such as transcription factors, as well as histones appears to be involved in this process. Acetylation may result in structural transitions as well as specific signaling within discrete chromatin domains. The role of acetylation in intracellular signaling has been inferred from the binding of acetylated peptides by the conserved bromodomain. Furthermore, recent findings suggest that bromodomain/acetylated-lysine recognition can serve as a regulatory mechanism in protein-protein interactions in numerous cellular processes such as chromatin remodeling and transcriptional activation.

Inmunógeno

Acetyl lysine-BSA corresponding to all species.

Aplicación

Anti-Acetyl Lysine, Pan, clone RM101 is an antibody against Acetyl Lysine, Pan for use in WB, Dot Blot, ICC, IP.

Immunocytochemistry Analysis: A representative lot detected Acetyl Lysine, Pan in A431 cells.
Dot Blot Analysis: A 1:2,000 dilution from a representative lot detected Acetyl Lysine, Pan in an Absurance Histone H3 Antibody Specificity Array (Cat. No. 16-667) and an Absurance Histone H2A, H2B, H4 Antibody Specificity Array (Cat. No. 16-665).
Immunoprecipitation Analysis: A 1:500 dilution from a representative lot immunoprecipitated Acetyl Lysine, Pan in A431 cell lysate.

Research Category
Epigenetics & Nuclear Function

Research Sub Category
Nuclear Receptors

Calidad

Evaluated by Western Blotting in Trichostatin A (TSA) treated HeLa cell lysate.

Western Blotting Analysis: A 1:2,000 of this antibody detected Acetyl Lysine, Pan in Trichostatin A (TSA) treated HeLa cell lysate.

Forma física

Protein A purified

Purified rabbit monoclonal IgG in buffer containing PBS with 1% BSA and 0.09% sodium azide.

Almacenamiento y estabilidad

Stable for 1 year at 2-8°C from date of receipt.

Otras notas

Concentration: Please refer to lot specific datasheet.

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Información de seguridad

• Código de clase de almacenamiento: 12 - Non Combustible Liquids
• Clase de riesgo para el agua (WGK): WGK 2
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable