MABC1690M

Anti-RAINBOW

from mouse

• UNSPSC Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Propiedades

• biological source: mouse
• Quality Level: 100
• antibody form: purified antibody
• antibody product type: primary antibodies
• clone: monoclonal
• species reactivity (predicted by homology): all
• packaging: antibody small pack of 25 μL
• technique(s): western blot: suitable
• isotype: IgG1κ
• target post-translational modification: unmodified

Descripción

General description

Anti-RAINBOW Antibody, Cat. No. MABC1690-25UL, validated for use in Western Blotting.

Pre-stained proteins are commonly used as molecular weight markers in electrophoresis. Several protein markers are available as pre-stained proteins with vinyl sulfone dyes. After separation through electrophoresis, the protein of interest is visualized by specific antibody coupled to a fluorophore or by chemiluminescence detection system. Anti-RAINBOW antibody is a unique antibody that can detect pre-stained molecular markers from different vendors without the need to perform a manual marking and estimating molecular weights. Anti-RAINBOW antibody detects all marker proteins in commercially available RAINBOW markers tested and lacks cross-reactivity with unstained proteins in whole cell lysates of a variety of species. It displays equal detection of Remazol-dye stained proteins: phosphorylase b (red), BSA and ADH (blue), chymotrypsin (yellow) and lysozyme (orange).

Specificity

Anti-RAINBOW is a mouse monoclonal antibody that can be used as a universal tool for visualizing prestained protein molecular weight markers in Western blot analysis. It recognizes and cross-reacts with comparable affinity with four different Remazol dye immunogens.

Immunogen

Remazol-dyes phosphorylase b (red), BSA and ADH (blue), chymotrypsin (yellow) and lysozyme (orange).

Application

Anti-RAINBOW, Cat. No. MABC1690, is a mouse monoclonal antibody that recognizes four different Remazol dye-conjugated proteins and can be used to visualize prestained molecular weight markers in Western Blotting.

Western Blotting Analysis: A representative lot detected Remazol-dye stained protein markers in Western Blotting applications (Schuchner, S., et. al. (2016). Sci Rep. 6:31363).

Western Blotting Analysis: A 1:250 and 1:500 dilution from a representative lot detected Remazol-dye Pre-Stained protein standards.

Quality

Evaluated by Western Blotting in Pre-Stained protein standard. Western Blotting Analysis: A 1:1,000, 1:5,000, and 1:10,000 dilution of this antibody detected Remzaol-dye prestained protein standards .

Physical form

Format: Purified

Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine, 150 mM NaCl, pH 7.4, with 0.05% sodium azide.

Other Notes

Concentration: Please refer to lot specific datasheet.

Información de seguridad

• Storage Class: 12 - Non Combustible Liquids
• wgk_germany: WGK 1