MAB4401

Anti-Oct-4 Antibody, clone 10H11.2

clone 10H11.2, Chemicon^®, from mouse

• Sinónimos: Octamer-binding transcription factor 3, POU class 5 homeobox 1, POU domain class 5, transcription factor 1, POU domain, class 5, transcription factor 1, POU-type homeodomain-containing DNA-binding protein, octamer-binding transcription factor-3
• Código UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Propiedades

• origen biológico: mouse
• forma del anticuerpo: purified antibody
• clon: 10H11.2, monoclonal
• reactividad de especies: human
• fabricante / nombre comercial: Chemicon^®
• técnicas: ELISA: suitable; flow cytometry: suitable; immunocytochemistry: suitable; western blot: suitable
• entrada: sample type induced pluripotent stem cell(s); sample type: human embryonic stem cell(s)
• isotipo: IgG1
• Nº de acceso NCBI: NM_002701.4
• Nº de acceso UniProt: Q01860
• Condiciones de envío: wet ice
• modificación del objetivo postraduccional: unmodified
• Información sobre el gen: human ... POU5F1(5460)

Descripción

Descripción general

Octamer-4 (Oct-4), a member of the POU family of transcription factors, has been demonstrated to be vital for the formation of self-renewing pluripotent stem cells. During embryogenesis expression of Oct-4 is limited to pluripotent cells of the inner cell mass (ICM) that contribute to the formation of all fetal cell types. This relationship between Oct-4 and pluripotency has seen this transcription factor emerge as a marker of pluripotent stem cells. Undifferentiated human and murine pluripotent Embryonic Stem (ES) and Embryonic Carcinoma (EC) cells express Oct-4. Additionally, murine Embryonic Germ (EG) cells are also known to express Oct-4. Following stem cell differentiation, the level of Oct-4 expression decreases. Oct-4 expression by human EG cells is not known.

Purified monoclonal antibody for the detection of Human Oct-4.

Especificidad

Reacts with human Oct-4 only, and detects the Oct-4A isoform. (Note: Please order Catalog No. MAB4419 for use with mouse embryonic stem cells).

The clone reacts strongly with specific nuclear binding to human ES cells and cross-reacts weakly with mouse ES cells.

Aplicación

Detect Oct-4 using this Anti-Oct-4 Antibody, clone 10H11.2 validated for use in WB, FC, IC, ELISA.

Flow Cytometry: A previous lot of this antibody was used in FC.

ELISA: A previous lot of this antibody was used in ELISA.

Immunocytochemistry: A previous lot of this antibody was used in IC. H9 cells in culture on a mouse embronic fibroblast feeder layer were labeled by a standard indirect IF protocol. Oct-4 labelling in green, DAPI in blue, & SSEA-3 (MAB4303 in red).

Optimal working dilutions must be determined by the end user.

Calidad

Evaluated by western blot on mouse P1 brain lysates.

Western Blotting: 1:500 dilution of this antibody detected Oct-4 on 10 µg of mouse P1 brain lysates

Descripción de destino

44 kDa

Forma física

Format: Purified

Nota de análisis

Control
Human embryonic stem cell lysate.

Otras notas

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Información legal

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Información de seguridad

• Código de clase de almacenamiento: 12 - Non Combustible Liquids
• Clase de riesgo para el agua (WGK): WGK 2
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable