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MAB3738

Sigma-Aldrich

Anti-PML Antibody, clone 36.1-104

ascites fluid, clone 36.1-104, Chemicon®

Sinónimos:

Promyelocytic Leukemia Protein, Tripartite Motif Protein 19

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About This Item

Código UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.41

origen biológico

mouse

forma del anticuerpo

ascites fluid

tipo de anticuerpo

primary antibodies

clon

36.1-104, monoclonal

reactividad de especies

mouse

no debe reaccionar con

human

fabricante / nombre comercial

Chemicon®

técnicas

immunocytochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable

isotipo

IgG2b

Nº de acceso UniProt

Condiciones de envío

dry ice

modificación del objetivo postraduccional

unmodified

Información sobre el gen

human ... PML(5371)

Descripción general

PML protein is a tripartite motif (TRIM)-containing nuclear protein that may function as, among other things, a transcription factor, a coactivator of nuclear receptors (Ruggerio, 2000), a regulator of apoptosis (Guo, 2000), a mediator of interferon-induced antiviral response (Regand and Chelbi-Aliz, 2001), and as a suppressor of growth and oncogenic transformation (Mu, 1997). PML is localized to the nucleoplasm and in distinct subnuclear structures referred to as Promyelocytic Leukemia Bodies (also known as Nuclear Domain 10). Localization of PML to Promyelocytic Leukemia Bodies requires modification of PML protein by the Small Ubiquitin Modifier (SUMO) and is required for proper formation and integrity of these subnuclear structures. At least 14 splice variants of PML ranging in molecular weight from 48-97 kDa (predicted) have been described in the literature. The functional significance of the various splice variants is not well understood. In patients with Acute Promyelocytic Leukemia, the PML gene is involved in at least two specific chromosomal translocations that result in the expression of chimeric proteins with the Retinoic Acid Receptor alpha (RARalpha DNA- and Hormone-binding domains; Pandolfi, 2001). All isoforms of PML, as well as the PML-RARalpha chimeric proteins expressed in Type A and Type B APL contain an identical N-terminus but vary in the C-terminal portion of the protein (Jenson, 2001).

Especificidad

Recognizes mouse PML (Promyelocytic Leukemia protein). On western blots of protein extracts from mouse embryo fibroblast (MEF1 cells), MAB3738 recognizes a band migrating at approximately 106 kDa corresponding to PML.

Inmunógeno

His-tagged PML fusion protein corresponding to amino acids 1-581 of mouse PML.

Aplicación

Anti-PML Antibody, clone 36.1-104 is a Mouse Monoclonal Antibody for detection of PML also known as Promyelocytic Leukemia Protein & has been validated in ICC, IP & WB.
Research Category
Epigenetics & Nuclear Function
Research Sub Category
Transcription Factors
Western blot (1:500)

Immunoprecipitation

Immunocytochemistry (1:100)

Optimal working dilutions must be determined by end user.

Descripción de destino

106 kDa

Forma física

Ascites fluid containing no preservatives.
Unpurified

Almacenamiento y estabilidad

Maintain for 1 year at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Nota de análisis

Control
POSITIVE CONTROL: Mouse embryo fibroblasts (MEF1 cells).

Otras notas

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Información legal

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Opcional

Referencia del producto
Descripción
Precios

Código de clase de almacenamiento

10 - Combustible liquids

Clase de riesgo para el agua (WGK)

WGK 1

Punto de inflamabilidad (°F)

Not applicable

Punto de inflamabilidad (°C)

Not applicable


Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

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Arkadiy K Golov et al.
PloS one, 10(10), e0139855-e0139855 (2015-10-06)
The extremely high concentration of macromolecules in a eukaryotic cell nucleus indicates that the nucleoplasm is a crowded macromolecular solution in which large objects tend to gather together due to crowding forces. It has been shown experimentally that crowding forces
Tracy Dagher et al.
The Journal of experimental medicine, 218(2) (2020-10-20)
Interferon α (IFNα) is used to treat JAK2V617F-driven myeloproliferative neoplasms (MPNs) but rarely clears the disease. We investigated the IFNα mechanism of action focusing on PML, an interferon target and key senescence gene whose targeting by arsenic trioxide (ATO) drives
Marie-Odile Baudement et al.
Genome research, 28(11), 1733-1746 (2018-10-06)
The mammalian cell nucleus contains numerous discrete suborganelles named nuclear bodies. While recruitment of specific genomic regions into these large ribonucleoprotein (RNP) complexes critically contributes to higher-order functional chromatin organization, such regions remain ill-defined. We have developed the high-salt-recovered sequences-sequencing
Sonia Missiroli et al.
Cell reports, 16(9), 2415-2427 (2016-08-23)
The precise molecular mechanisms that coordinate apoptosis and autophagy in cancer remain to be determined. Here, we provide evidence that the tumor suppressor promyelocytic leukemia protein (PML) controls autophagosome formation at mitochondria-associated membranes (MAMs) and, thus, autophagy induction. Our in vitro and
Arkaitz Carracedo et al.
The Journal of clinical investigation, 122(9), 3088-3100 (2012-08-14)
Cancer cells exhibit an aberrant metabolism that facilitates more efficient production of biomass and hence tumor growth and progression. However, the genetic cues modulating this metabolic switch remain largely undetermined. We identified a metabolic function for the promyelocytic leukemia (PML)

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