MAB3580
Anti-Green Fluorescent Protein Antibody
Chemicon®, from mouse
Sinónimos:
GFP, eGFP
Iniciar sesiónpara Ver la Fijación de precios por contrato y de la organización
About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Productos recomendados
biological source
mouse
Quality Level
antibody form
affinity purified immunoglobulin
antibody product type
primary antibodies
clone
monoclonal
species reactivity (predicted by homology)
all
manufacturer/tradename
Chemicon®
technique(s)
ELISA: suitable
immunocytochemistry: suitable
immunohistochemistry: suitable
western blot: suitable
isotype
IgG1
UniProt accession no.
target post-translational modification
unmodified
General description
The Green Fluorescent Protein (GFP) from the jellyfish Aequorea victoria is used as a fluorescent indicator for monitoring gene expression in a variety of cellular systems, including living organisms and fixed tissues. Unlike other bioluminescent reporters, GFP fluoresces in the absence of substrates, cofactors, or other intrinsic or extrinsic proteins. Purified GFP is a 27 kDa monomer consisting of 238 amino acids and emits green light (emission maximum at 509 nm) when excited with blue or UV light.
Specificity
Reacts with Green Fluorescent Protein (GFP). The antibody reacts with various forms of GFP including eGFP including those found in vectors supplied by Clonetech and Invitrogen. There is not cross-reactivity to RFP. YFP has not been tested. This antibody works well for immunohistochemistry.
Immunogen
GFP-BSA
Application
Detect Green Fluorescent Protein using this Anti-Green Fluorescent Protein Antibody validated for use in ELISA, IC, IH & WB.
Western blot: 1:2,500-1:5,000. Recommended dilution buffer is PBS containing 1 mg/mL BSA.
Immunocytochemistry: 1:500-1:1,000
Immunohistochemistry: 1:500-1:1,000 on paraformaldehyde fixed tissues with an overnight incubation using Nickel enhanced DAB for detection. Suggested permeabilization method is 0.5% Triton X-100. Recommended blocking buffer is PBS containing BSA and serum from the host of the secondary antibody. Recommended dilution buffer is PBS containing 1 mg/mL of BSA.
ELISA: 1:1,000-1:2,000 in a direct assay.
Optimal working dilutions must be determined by the end user.
Immunocytochemistry: 1:500-1:1,000
Immunohistochemistry: 1:500-1:1,000 on paraformaldehyde fixed tissues with an overnight incubation using Nickel enhanced DAB for detection. Suggested permeabilization method is 0.5% Triton X-100. Recommended blocking buffer is PBS containing BSA and serum from the host of the secondary antibody. Recommended dilution buffer is PBS containing 1 mg/mL of BSA.
ELISA: 1:1,000-1:2,000 in a direct assay.
Optimal working dilutions must be determined by the end user.
Target description
MW for GFP is 27 kDa Dependent upon the molecular weight of the fusion protein being detected.
Physical form
Format: Purified
Liquid in PBS containing 1 mg/mL BSA. Contains no preservative.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Not finding the right product?
Try our Herramienta de selección de productos.
Optional
Referencia del producto
Descripción
Precios
Storage Class
12 - Non Combustible Liquids
wgk_germany
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificados de análisis (COA)
Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»
¿Ya tiene este producto?
Encuentre la documentación para los productos que ha comprado recientemente en la Biblioteca de documentos.
Los clientes también vieron
Drosophila Dpp morphogen movement is independent of dynamin-mediated endocytosis but regulated by the glypican members of heparan sulfate proteoglycans.
Belenkaya, TY; Han, C; Yan, D; Opoka, RJ; Khodoun, M; Liu, H; Lin, X
Cell null
Multiple phenotypes resulting from a mutagenesis screen for pharynx muscle mutations in Caenorhabditis elegans.
Ferrier, A; Charron, A; Sadozai, Y; Switaj, L; Szutenbach, A; Smith, PA
Testing null
Magdalena Jasińska et al.
Molecular neurobiology, 53(7), 4701-4712 (2015-09-01)
Mir-132 is a neuronal activity-regulated microRNA that controls the morphology of dendritic spines and neuronal transmission. Similar activities have recently been attributed to matrix metalloproteinase-9 (MMP-9), an extrasynaptic protease. In the present study, we provide evidence that miR-132 directly regulates
Benjamin J Spink et al.
Nature structural & molecular biology, 15(6), 591-597 (2008-05-31)
Myosin VI has challenged the lever arm hypothesis of myosin movement because of its ability to take approximately 36-nm steps along actin with a canonical lever arm that seems to be too short to allow such large steps. Here we
Jennifer J Johnston et al.
American journal of human genetics, 97(3), 465-474 (2015-08-19)
Penttinen syndrome is a distinctive disorder characterized by a prematurely aged appearance with lipoatrophy, epidermal and dermal atrophy along with hypertrophic lesions that resemble scars, thin hair, proptosis, underdeveloped cheekbones, and marked acro-osteolysis. All individuals have been simplex cases. Exome
Nuestro equipo de científicos tiene experiencia en todas las áreas de investigación: Ciencias de la vida, Ciencia de los materiales, Síntesis química, Cromatografía, Analítica y muchas otras.
Póngase en contacto con el Servicio técnico